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We narrowed to 533 results for: plasmid cloning by pcr

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Showing: 181 - 200 of 533 results

  1. pTF261 (pFA6a-TEV-6xGly-HphMX)

    Plasmid
    #44080
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44080-plasmid-map-sequence-id-61664

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  2. pTF262 (pFA6a-PSc-6xGly-HphMX)

    Plasmid
    #44074
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44074-plasmid-map-sequence-id-61665

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  3. pTF259 (pFA6a-TEV-6xGly-KanMX)

    Plasmid
    #44092
    Depositor
    Tim Formosa
    Type
    Empty backbone
    Use
    Pcr-based yeast c-terminal tagging
    Tags
    (XhoI-KpnI-XbaI)-containing spacer, 6xGly linker,…
    Available Since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    44092-plasmid-map-sequence-id-61662

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  4. pECT5

    Plasmid
    #75451
    Purpose
    Plasmid that serves as the PCR template to create genomic pdt5 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    pdt5
    Expression
    Bacterial
    Promoter
    none
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75451-plasmid-map-sequence-id-146479

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  9. pATF1-donor

    Plasmid
    #112328
    Purpose
    CRISPR donor plasmid to tag human transcription factor ATF1 with GFP
    Depositor
    Kevin White
    Insert
    ATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
    Use
    CRISPR
    Mutation
    hg38:12:50819876:T>A, hg38:12:50819888:T>C,…
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112328-plasmid-map-sequence-id-228551

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  11. pattB-aubergine-ADH-gf

    Plasmid
    #69448
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
    Depositor
    Phillip Zamore
    Insert
    aubergine (aub Fly)
    Tags
    NA
    Expression
    Insect
    Mutation
    chr2L:10,998,626(−)T>G
    Promoter
    aubergine
    Available Since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69448-plasmid-map-sequence-id-122550

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  12. GLRX3

    Plasmid
    #39036
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GLRX3 (GLRX3 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    A PCR artifact adds a C-terminal sequence includi…
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. LHP1034 - ECFP-Nterm

    Plasmid
    #32436
    Depositor
    Linda Hicke
    Insert
    ECFP
    Use
    Cloning
    Available Since
    Oct. 21, 2011
    Availability
    Academic Institutions and Nonprofits only

  18. LHP1032 - EYFP-Nterm

    Plasmid
    #32438
    Depositor
    Linda Hicke
    Insert
    EYFP
    Use
    Cloning
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. LHP1033 - EYFP-Cterm

    Plasmid
    #32437
    Depositor
    Linda Hicke
    Insert
    EYFP
    Use
    Cloning
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. LHP1035 - ECFP-Cterm

    Plasmid
    #32435
    Depositor
    Linda Hicke
    Insert
    ECFP
    Use
    Cloning
    Available Since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 181 - 200 of 533 results