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pKM631

Plasmid

#17727

Depositor

David Waugh

Article

Kapust et al Biochem Biophys Res Commun. 2002 Jun 28.

Insert

NusG (nusG Aquifex aeolicus)

Tags

His6 and MBP

Expression

Bacterial

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pIMAY*

Plasmid

#121441

Purpose

Allelic exchange plasmid with PheS* counter-selection for Staphylococcus aureus

Depositor

Angelika Grundling

Article

Schuster et al Microbiology. 2019 Apr 3. doi: 10.1099/m

Type

Empty backbone

Use

Escherichia coli/staphylococcus aureus shuttle ve…

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRK603

Plasmid

#8831

Depositor

David Waugh

Article

Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

Insert

TEV protease, S219D mutant

Expression

Bacterial

Mutation

S219D mutant

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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HyGCaMP6s

Plasmid

#102558

Purpose

Expression of GCaMP6s in Hydra

Depositor

Rafael Yuste

Article

Dupre et al Curr Biol. 2017 Apr 24;27(8):1085-1097.

Insert

GCaMP6s

Use

Hydra expression

Tags

Actin/GCaMP6s fusion protein (C terminal on backb…

Available Since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

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p1177-X1-8

Plasmid

#38150

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2011 May;77(1):53-61

Insert

Carboxypeptidase A

Tags

HHHHHHRR

Expression

Insect

Promoter

polyhedron

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pMOTag-glmS-4H

Plasmid

#106378

Purpose

Can be used as a PCR template to amplify the glmS-HA fragment for gene specific tagging and regulation

Depositor

Roberto Docampo

Article

Cruz-Bustos et al J Eukaryot Microbiol. 2017 Dec 19. doi:

Insert

GlmS-3xHA

Available Since

March 2, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pFTG5

Plasmid

#149487

Purpose

Broad-Host range expression vector, RSF1010 origin, TetR repressor, PTetA promoter, mRFP.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFTtG5

Plasmid

#149488

Purpose

Broad-Host range expression vector, RSF1010 origin, TtgR(AM) repressor, PTtg promoter, mRFP.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TtgR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTtg

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWLlR5

Plasmid

#149492

Purpose

Broad-Host range expression vector, incW (pSa) origin, LacI repressor, PLlacO-1 promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LacI-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLlacO-1

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pmEGFP-DRD2

Plasmid

#190752

Purpose

Expression of DRD2 with mEGFP tag on N-terminus.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

DRD2 (DRD2 Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFNR5

Plasmid

#149483

Purpose

Broad-Host range expression vector, RSF1010 origin, NahR(AM) repressor, PSalTTC promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PSalTTC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKCyR8

Plasmid

#149494

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFLxR3

Plasmid

#167518

Purpose

Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pFCiR2

Plasmid

#167512

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pBLxG2

Plasmid

#149472

Purpose

Broad-Host range expression vector, pBBR origin, LuxR activator, PLuxB promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKCyR6

Plasmid

#149493

Purpose

Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Spectinomycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymR(AM)-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBTG2

Plasmid

#149475

Purpose

Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

TetR-GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTetA

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFCyR2

Plasmid

#167513

Purpose

Broad-Host range expression vector, RSF1010 origin, CymRAM repressor, PCymRC promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CymRAM-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCymRC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFCiKm7

Plasmid

#167521

Purpose

Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

CinRAM-KmR

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCin

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pFVK5

Plasmid

#167522

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-mKelly2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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