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pEF myc-SHP2(L43F)

Plasmid

#245224

Purpose

Mammalian expression of myc-tagged full-length SHP2

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

SHP2 full-length

Tags

myc

Expression

Mammalian

Mutation

L43F

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pWJ 1781 URA c-Src KD

Plasmid

#245256

Purpose

Expression of the kinase domain of c-Src in yeast

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Insert

Kinase domain of c-Src

Expression

Yeast

Mutation

wild-type

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEM1_Euk_2NLS-WT ( LbCas12a)-2NLS

Plasmid

#244836

Purpose

Mammalian expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM4_CL7_Bac_2NLS-WT (LbCas12a)-4NLS

Plasmid

#244839

Purpose

Bacterial expression of wild-type LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM8_2CTH10_Bac_Lbm6 (LbCas12a)-4NLS

Plasmid

#244843

Purpose

Bacterial expression of Lbm6-LbCas12a with 10xHis-MBP at N-temrinus and 4xSV40 NLS at C-temrinus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Lbm6-Cas12a

Use

CRISPR

Tags

10xHis-MBP and 4xSV40 NLS

Expression

Bacterial

Mutation

D535G/S551F/D665N

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_H1-5

Plasmid

#223820

Purpose

Bacterial expression of SynPspA H1-5 with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

truncation of helix 0

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA

Plasmid

#223818

Purpose

Bacterial expression of SynPspA with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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PFN29A-DDX6

Plasmid

#240019

Purpose

Expresses human DDX6 fused to EGFP in bacterial cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

DDX6

Expression

Bacterial

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgCNOT3-1

Plasmid

#240008

Purpose

Expresses human sgCNOT3 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgCNOT3

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgDDX6-2

Plasmid

#240005

Purpose

Expresses human sgDDX6 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgDDX6

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgPABPC1-1

Plasmid

#240007

Purpose

Expresses human sgPABPC1 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgPABPC1

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgEIF4E-1

Plasmid

#240006

Purpose

Expresses human sgEIF4E in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgEIF4E

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-hygro-sgDDX6-1

Plasmid

#240004

Purpose

Expresses human sgDDX6 in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

sgDDX6

Use

Lentiviral

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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BtA1N

Plasmid

#238979

Purpose

In vitro transcription of bovine arrestin-1

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-1 (SAG Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2N

Plasmid

#238980

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINS-HOIP NZF1 (351-379)

Plasmid

#235655

Purpose

Bacterial expression of human HOIP NZF1 (351-379)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

HOIP NZF1 (RNF31 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 351-379 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-ZRANB3 NZF (619-650)

Plasmid

#235654

Purpose

Bacterial expression of human ZRANB3 NZF (619-650)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

ZRANB3 NZF (ZRANB3 Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 619-650 only

Promoter

T7

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINK-Sharpin NZF (347-376)

Plasmid

#235651

Purpose

Bacterial expression of human Sharpin NZF (347-376)

Depositor

David Komander

Article

Michel et al Cell Rep. 2024 Aug 27;43(8):114545. doi:

Insert

Sharpin NZF (SHARPIN Human)

Tags

6xHis-GST-3c

Expression

Bacterial

Mutation

aa 347-376 only

Promoter

T7

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSF503

Plasmid

#222587

Purpose

BB3 gsdA tet-on overexpression cassette with homologous flanks for integration at pyrG locus

Depositor

Matthias Steiger

Article

Matthias et al PLOS One

Insert

gsdA (gsdA )

Use

A. niger

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert,…

Promoter

tet-on

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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SpyCatcher-P-Domain

Plasmid

#201193

Purpose

Expresses furin P-domain genetically fused to SpyCatcher001 in bacteria

Depositor

Vesa Hytönen

Article

Lampinen et al Nanoscale Adv. 2024 Oct 9;6(24):6239-52.

Insert

SpyCatcher-P-Domain

Tags

HisTag and SpyCatcher001

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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