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pYL116

Plasmid

#239967

Purpose

CRISPR donor plasmid with Puro-T2A-mCherry cassette for Suz12 disruption

Depositor

Thomas Cech

Article

Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

Insert

PuroR-T2A-mCherry with homology arms for human Suz12

Use

CRISPR

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYL117

Plasmid

#239968

Purpose

CRISPR donor plasmid with Puro-T2A-mCherry cassette for EZH2 disruption

Depositor

Thomas Cech

Article

Long et al Methods. 2021 Jul;191:44-58. doi: 10.101

Insert

PuroR-T2A-mCherry with homology arms for human Ezh2

Use

CRISPR

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pVID

Plasmid

#236635

Purpose

CRISPR cloning, gRNA expression, and detection of undesired vector insertion during HDR for Drosophila

Depositor

Brian McCabe

Article

Källstig et al bioRxiv 2025.08.11.669658; doi: 10.1101/

Type

Empty backbone

Use

CRISPR

Promoter

dU6:3

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pUC57 β-Globin

Plasmid

#194437

Purpose

In Situ hybridization probes

Depositor

Christopher Sansam

Article

Masser et al eLife 2023 (Reviewed Preprint) 12:RP8767

Insert

β-globin

Use

In situ hybridization

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAB228i16

Plasmid

#209738

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[mCNPR43] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228i24

Plasmid

#209739

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[pCNPR02] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228r3

Plasmid

#209741

Purpose

ColA BBa_B0014 tetAp SD8 Lum1PylRS-[PylHRS*] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

Mlum1RS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228p

Plasmid

#209742

Purpose

ColA BBa_B0014 tetAp SD8 MmPylRS-[wt] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228p4

Plasmid

#209743

Purpose

ColA BBa_B0014 tetAp SD8 MmPylRS-[AF] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

MmPylRS

Expression

Bacterial

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pETURALEU

Plasmid

#223207

Purpose

Plasmid for selection/counterslection in budding yeast, containing URA3 and LEU2 markers

Depositor

Sahand Jamal Rahi

Article

Stojkovic et al G3 (Bethesda). 2024 Dec 17:jkae297. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[GFP]

Plasmid

#213903

Purpose

Mariner transposon expressing mNeonGreen with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and mNeonGreen

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[RFP]

Plasmid

#213904

Purpose

Mariner transposon expressing mCherry with cam selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and mCherry

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCJ164

Plasmid

#175057

Purpose

rbcLXS7002 integrative plasmid expresses DRE recombinase as a translational fusion to rbcL

Depositor

David Nielsen

Article

Jones et al ACS Synth Biol. 2021 Sep 17;10(9):2371-2

Insert

DRE

Use

Unspecified

Promoter

rbcLXS

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS621

Plasmid

#188893

Purpose

ttTi5605 MosSci targeting vector with floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Type

Empty backbone

Use

Mossci targeting vector

Available Since

Aug. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pFH43

Plasmid

#126020

Purpose

Level0 Pea RbSC E9 terminator

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Pea RbSC E9 terminator

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH48

Plasmid

#125813

Purpose

Level0 Csy4

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Csy4

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH40

Plasmid

#125879

Purpose

Level0 Switchgrass PvUBI1 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Switchgrass PvUbi1 Pol II promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH15

Plasmid

#125800

Purpose

Level0 StCas9, Arabidopsis codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SaCas9, Arabidopsis codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH46

Plasmid

#125811

Purpose

Level0 FnCas12a, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

FnCas12a, human codon optimized

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRVV610

Plasmid

#119018

Purpose

RMCE vector containing inverted TP901-1 attB sites flanking large MCS

Depositor

Richard Mann

Article

Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

Insert

TP901-1 attB sites

Use

Fruit fly rmce

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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