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Plasmid#71685PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A Synthetic, S. pyogenes, Human)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-EGFPExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)_v2
Plasmid#74408PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (v2) (DNMT3A Synthetic, S. pyogenes, Human)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF219_1
Plasmid#72368PurposeEncodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFPDepositorInsertZNF219 (ZNF219 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF219_2
Plasmid#72369PurposeEncodes gRNA for 3' target of human ZNF219 along with Cas9 with 2A GFPDepositorInsertZNF219 (ZNF219 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOTX2-donor
Plasmid#176358PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertOTX2 (OTX2 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSTAT5B-donor
Plasmid#176339PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertSTAT5B (STAT5B Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_ZNF146_1
Plasmid#72360PurposeEncodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A GFPDepositorInsertZNF146 (ZNF146 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOSR1-donor
Plasmid#176363PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertOSR1 (OSR1 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF627-donor
Plasmid#176365PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF627 (ZNF627 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF608-donor
Plasmid#176362PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF608 (ZNF608 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZNF79-donor
Plasmid#176371PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF79 (ZNF79 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZBTB4-donor
Plasmid#176374PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZBTB4 (ZBTB4 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSNAPC5-donor
Plasmid#176341PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertSNAPC5 (SNAPC5 Human)
UseCRISPRTagsGFPExpressionMutationPromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHAP12-N1.1.2-gDNA
Plasmid#175860PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTHAP12-Nickase2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHCHD3-N2.1.2-gDNA
Plasmid#175858PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCHCHD3-Nickase2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCHCHD3-N1.1.1-gDNA
Plasmid#175857PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCHCHD3-Nickase1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTSHZ1-donor
Plasmid#132479PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertTSHZ1 (TSHZ1 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF449-donor
Plasmid#132515PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF449 (ZNF449 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF8-donor
Plasmid#136689PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertZNF8 (ZNF8 Human)
UseCRISPRTagsGFPExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZNF132.1.0-gDNA
Plasmid#132468PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertZNF132 (ZNF132 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only