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NHR1+2-Flag pBacPAK8-3

Plasmid

#17324

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-OSBP PH domain

Plasmid

#58840

Purpose

Expresses GFP-Tagged OSBP PH domain in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
L1-1180

Plasmid

#13265

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

Truncated after RSLE region

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
Ctcf-Halo-mAID donor

Plasmid

#113103

Purpose

Donor plasmid for knock-in mAID-Halo fusion to the c-terminal of mouse Ctcf coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

c terminal Ctcf donor region including homology arms and mAID-HaloTag (Ctcf Synthetic, Mouse)

Use

Synthetic Biology

Available Since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mScarlet-H2BC11

Plasmid

#207756

Purpose

Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-FKBP-SAC1-HLx8

Plasmid

#108136

Purpose

Recruitable SAC1 with extended linker

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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AA008

Plasmid

#216003

Purpose

Fragmid fragment: (Cas protein) preferred over wildtype Cas12a

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas12a_v1.1 [EnAs]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-MYO1C

Plasmid

#227305

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of MYO1C (MYO1C Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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cpGFP-Adhiron_flex

Plasmid

#165044

Purpose

Flexible linker control for POLArISact expression in mammalian cells

Depositor

Sumio Terada

Article

Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

Insert

cpGFP-Adhiron (connected with a flexible linker)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-uTEV1-MAVS

Plasmid

#170998

Purpose

HiLITR protease with MAVS c-terminal tmd targeting information (Mitochondria)

Depositor

Alice Ting

Article

Coukos et al Elife. 2021 Aug 20;10. pii: 69142. doi:

Insert

EGFP-uTEV1-MAVS(tmd)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

pTRE-tight (TetOn)

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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