Addgene

We narrowed to 17,993 results for: Comp

Showing: 2001 - 2020 of 17993 results

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pET21d-colEdes2/Imdes2

Plasmid

#107109

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des2

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes2/Imdes2

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes3/Imdes3

Plasmid

#107110

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3/Imdes3

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes3.5/Imdes3.5

Plasmid

#107111

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3.5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3.5/Imdes3.5

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes4/Imdes4

Plasmid

#107112

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des4

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes4/Imdes4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes5/Imdes5

Plasmid

#107113

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des5

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes5/Imdes5

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21d-colEdes6/Imdes6

Plasmid

#107114

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des6

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes6/Imdes6

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB

Plasmid

#80610

Purpose

pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Arp2/3_pBIG2abcd

Plasmid

#173683

Purpose

Insect expression of the 7-subunit Human Arp2/3 complex

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Inserts

Arp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a

Plasmid

#80611

Purpose

pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-His-PheRS

Plasmid

#124116

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pVP143

Plasmid

#222080

Purpose

Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and AeBlue for plasmid eviction.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

Insert

dCas9-PmCDA1, sfGFP, sacB and AeBlue

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pVP144

Plasmid

#222081

Purpose

Single component CRISPR-mediated base-editor for Agrobacterium genetic engineering visually marked with sfGFP for guide insertion and eforRED for plasmid eviction.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 March, 7:100001. doi: 1

Insert

dCas9-PmCDA1, sfGFP, sacB and eforRED

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-GPI

Plasmid

#65294

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-EGFP-GPI

Plasmid

#65298

Purpose

synchronize trafficking of EGFP-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-raptor

Plasmid

#73385

Purpose

Expresses YFP and HA tagged Human raptor

Depositor

Taekjip Ha, Jie Chen

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsSmg5_T

Plasmid

#147552

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-mCherry-raptor

Plasmid

#73386

Purpose

Expresses raptor tagged with mCherry and HA

Depositor

Taekjip Ha, Jie Chen

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Use

Tags

HA and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Str-KDEL_SBP-mCherry-Ecadherin

Plasmid

#65287

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Use

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge