Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 18,510 results for: puro

Clear keywords
Showing: 2001 - 2020 of 18510 results

  4. pAK-DR30(SapI)-CasRx-Puro-pA/EF1A-mCherry-WPRE-pA

    Plasmid
    #233045
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx and a Puro resistance gene. It also encodes an mCherry gene. The CasRx and Puro coding regions are separated by a T2A site.
    Depositor
    Jonathan Ploski
    Article
    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx and mCherry
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233045-plasmid-map-sequence-id-467425

    Enlarge

  8. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

    Enlarge

  10. pLV U6 sgRNA2.0 pp7 EFS Puro 2A.BFP

    Plasmid
    #255463
    Purpose
    Expresses Puro and BFP and has an sgRNA2.0 scaffold for insertion of CRISPR activation gRNAs
    Depositor
    Le Cong
    Type
    Empty backbone
    Expression
    Mammalian
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    255463-plasmid-map-sequence-id-508841

    Enlarge

  13. pKAM-ME-Flag-BFP

    Plasmid
    #101868
    Purpose
    Lentiviral Mds1-Evi-1-Flag with dual BFP and puro markers
    Depositor
    Archibald Perkins
    Insert
    Mds1-Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    101868-plasmid-map-sequence-id-211131

    Enlarge

  16. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    U6 and synthetic Probasin ARRx2
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68357-plasmid-map-sequence-id-122833

    Enlarge

  19. pTS2334_Tier3(Lenti_inverse)-rtTA-YPet_Puro

    Plasmid
    #169665
    Purpose
    Tier-3 vector for lentiviral stable integration of the rtTA and puromyine - Ypet selection cassette in inverted direction with polyA (PRPBSA-YPet-p2a-PuroR::PPGK-rtTA-pA::A3-pA)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK / RPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169665-plasmid-map-sequence-id-365640

    Enlarge

  20. pKAM-E-Flag-BFP

    Plasmid
    #101867
    Purpose
    Lentiviral Evi-1-Flag with dual BFP and puro markers
    Depositor
    Archibald Perkins
    Insert
    Evi-1 (Mecom Mouse)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    101867-plasmid-map-sequence-id-211116

    Enlarge

Showing: 2001 - 2020 of 18510 results