We narrowed to 9,077 results for: tet on

1. pAV-H1promoter-2xTetO-Tornado-2xALFA-10xSunTag

   Plasmid

   #231591

   Purpose

   Express circular RNA translation reporter with 2xALFATag-10xSunTag under H1 promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Tornado-2xALFATag-10xSunTag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

   Plasmid

   #112669

   Purpose

   Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

   Depositor

   Catherine Verfaillie

   Article

   Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

   Insert

   tdT
   

   Use

   AAV; Donor plasmid for recombinase-mediated casse…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. PiggyBac-TetO-En1-t2a-Foxa2-hygro

   Plasmid

   #176483

   Purpose

   TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   En1-t2a-FoxA2 (FOXA2, En1 Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

   Plasmid

   #113885

   Purpose

   Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L

   Plasmid

   #196136

   Purpose

   SB vector for the mammalian expression of GADD34-K3L

   Depositor

   Jamie Cate

   Article

   Aleksashin et al bioRxiv. 2023 Feb 10:2023.02.09.527910.

   Insert

   GADD34 Δ1-240 - P2A - K3L (PPP1R15A vaccinia virus, Human)
   

   Use

   Tags

   P2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fuw-dCas9-dead Tet1CD-P2A-BFP

   Plasmid

   #108246

   Purpose

   Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

   Insert

   dCas9-dead Tet1CD-HA-P2A-BFP
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

   Promoter

   Ubc

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBEST-p15a-pBAD-UTR1-TetR-T500

   Plasmid

   #45787

   Depositor

   Richard Murray

   Insert

   Tetracycline repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

   Plasmid

   #117687

   Purpose

   U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

   Depositor

   Erik Sontheimer

   Article

   Gao et al Methods Enzymol. 2019;616:365-383. doi:

   Inserts

   Kanamycin cassette
   TetR-P2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and hPGK

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PiggyBac-TetO-Lmx1a-t2a-Pitx3-puro

   Plasmid

   #176484

   Purpose

   TetO-Lmx1a-t2a-Pitx3 in a PiggyBac vector with puromycin resistance

   Depositor

   Marius Wernig

   Article

   Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

   Insert

   Lmx1A-T2A-Pitx3 (PITX3, LMX1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER

    Plasmid

    #113898

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. tetO-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid

    #69545

    Purpose

    Tet-On system expressing GFP tagged with 2x nuclear localization signals.

    Depositor

    Charles Gersbach

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2x nuclear localization sequence and T2A "sk…

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

    Plasmid

    #113888

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

    Plasmid

    #113899

    Purpose

    Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    TetR-HA-NLS-P2A-BSD-Myc
    

    Use

    Lentiviral

    Tags

    HA-Myc

    Expression

    Mutation

    Promoter

    CMV-MIE

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

    Plasmid

    #202571

    Purpose

    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

    Depositor

    Liam Holt

    Article

    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

    Insert

    LINE-1
    

    Use

    Tags

    GFP-AI retrotransposition reporter and HaloTag7 o…

    Expression

    Mammalian

    Mutation

    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

    Promoter

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

    Plasmid

    #113884

    Purpose

    Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Twin-Strep

    Expression

    Mutation

    Promoter

    pHR-CMV-TetO2

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)

    Plasmid

    #199763

    Purpose

    Elongation reporter construct to quantify elongation of LacZ

    Depositor

    Brian Zid

    Article

    Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

    Insert

    LacZ-nLucPEST
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TetO7

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

    Plasmid

    #199346

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-H1promoter-2xTetO-Tornado-1xALFA-5xSunTag

    Plasmid

    #231597

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200631

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    pTRE3G

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid

    #100597

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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