Addgene

We narrowed to 3,037 results for: ALC

Showing: 2021 - 2040 of 3037 results

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pLKO5d.EFS.SpCas9.P2A.BSD

Plasmid

#57821

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-BSD

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO5d.EFS.SpCas9.P2A.PAC

Plasmid

#58329

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S218D S222D

Promoter

Available since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
cacnb4 (rat) in pMT2 vector

Plasmid

#107426

Purpose

Expresses Calcium channel beta4 auxillary subunit in mammalian cells

Depositor

Annette Dolphin

Article

Brodbeck et al J Biol Chem. 2002 Mar 8;277(10):7684-93.

Insert

cacnb4 (Cacnb4 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ad MLP

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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Cherry-HA-KCC2

Plasmid

#104077

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Use

Tags

mCherry-HA tag

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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CD44-isoform1-CD4d3+4-bio

Plasmid

#73098

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD44 antigen isoform 1 (CD44 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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FUW-Flag-Mbd3

Plasmid

#52371

Purpose

Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

Ub

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-CAAX-SV40

Plasmid

#117815

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-H-RAS V12

Plasmid

#31201

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

H-Ras (HRAS Human)

Use

Gateway donor vector

Tags

Expression

Mutation

G12V constitutively active

Promoter

Available since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pGTag-TagRFP-CAAX-B-actin

Plasmid

#117810

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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REV human OCT4 TALEN

Plasmid

#52413

Purpose

TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

Depositor

Jacob Hanna

Article

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN

Use

TALEN

Tags

Expression

Mutation

Promoter

CAGGS

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only
pGTag-NLS-TagRFP-B-actin

Plasmid

#117806

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGTag-eGFP-CAAX-B-actin

Plasmid

#117816

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-Mbd3

Plasmid

#52356

Purpose

Lentiviral expression vector for constitutive expression of Mbd3

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

Mbd3 (Mbd3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pGTag-NLS-eGFP-B-actin

Plasmid

#117812

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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CD44-isoform12-CD4d3+4-bio

Plasmid

#73130

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding CD44-isoform12 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

CD44 antigen isoform 12 (CD44 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNOC_dfnCas12a-KRAB_crRNA NC

Plasmid

#176262

Purpose

pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

Kruppel associated box domain and luciferase

Expression

Mutation

D917A and E1006A mutations to inactivate the endo…

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-CAGGS-ERAS

Plasmid

#52416

Purpose

Lentiviral vector encoding constitutive expression of human ERAS cDNA

Depositor

Jacob Hanna

Article

Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

Insert

ERAS (ERAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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shCCL20

Plasmid

#65096

Purpose

retroviral expression of CCL20 shRNA

Depositor

Pau Sancho-Bru

Article

Affo et al Gut. 2014 Nov;63(11):1782-92. doi: 10.11

Insert

Ccl20 shRNA (Ccl20 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-NES-YFP-CCTd

Plasmid

#184326

Purpose

Mammalian expression of the cytosolic localized YFP-CCTd(peptide of Cav1.3)

Depositor

Xiaodong Liu

Article

Yang et al Commun Biol. 2022 May 19;5(1):484. doi:

Insert

CCTd (CACNA1D Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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