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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,219 results for: Lif

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Showing: 2021 - 2040 of 8219 results

  5. RNF169-Halo HRD

    Plasmid
    #207083
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available Since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207083-plasmid-map-sequence-id-432319

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  6. pCL1315

    Plasmid
    #217957
    Purpose
    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F84A, L92A, Y99A
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217957-plasmid-map-sequence-id-430211

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  7. pCL1314

    Plasmid
    #217956
    Purpose
    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between A95 and C96
    Promoter
    CMV
    Available Since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217956-plasmid-map-sequence-id-430210

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  8. pINER3R

    Plasmid
    #185883
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    185883-plasmid-map-sequence-id-430023

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  13. pETM11-6xHis-TEV-hORF1p_StammerAAA

    Plasmid
    #202587
    Purpose
    Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    ORF1p
    Tags
    6x His and TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…
    Available Since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    202587-plasmid-map-sequence-id-415633

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Showing: 2021 - 2040 of 8219 results