We narrowed to 6,214 results for: actin

1. pMH-SFB-DYRK1A

   Plasmid

   #101770

   Purpose

   Mammalian expression of DYRK1A fusion protein

   Depositor

   Michael Huen

   Article

   Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

   Insert

   DYRK1A (DYRK1A Human)
   

   Tags

   S peptide-flag-streptavidin binding peptide (SFB)

   Expression

   Mammalian

   Mutation

   siRNA-resistant allele

   Available Since

   Oct. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-Intersectin Long

   Plasmid

   #47395

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   intersectin long (ITSN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL3.7-EF-EYFP-YAP1_WT-PolyA

   Plasmid

   #112284

   Purpose

   Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Mutation

   WT human YAP1-2gamma

   Promoter

   EF-1 alpha

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-DeAct-SpvB

   Plasmid

   #89446

   Purpose

   Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Intersectin Short

   Plasmid

   #47394

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   Intersectin short (ITSN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-FLAG-Akt2-CA

   Plasmid

   #64050

   Purpose

   Myristoylated-FLAG-tagged Akt2, constitutively active

   Depositor

   Weiping Han

   Article

   Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

   Insert

   AKT2 (AKT2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG and MYR

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)

   Plasmid

   #174637

   Purpose

   Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVDfast-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
   

   Tags

   mVenus-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB80-KIF1A(1-365)-VVD52C-mVenus-SSPB(micro)

   Plasmid

   #174636

   Purpose

   Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   KIF1A(1-365)-VVD52C-mVenus-SSPB(micro) (Kif1a Mouse, Synthetic)
   

   Tags

   mVenus-SSPB(micro)

   Expression

   Mammalian

   Mutation

   mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

   Promoter

   Chicken beta-actin

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2/DEST/hCx43-E2HA-stop

   Plasmid

   #40909

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   Connexin 43 internal HA tag (GJA1 Human)
   

   Expression

   Mammalian

   Mutation

   HA tag inserted in second extracellular loop

   Promoter

   CMV

   Available Since

   Nov. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pRK5 Myc-tagged PSK

    Plasmid

    #197149

    Purpose

    Expression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

    Insert

    TAOK2 (TAOK2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    SV40

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)

    Plasmid

    #174630

    Purpose

    Poorly reversible opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVD52C-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTetON-DeAct-SpvB

    Plasmid

    #89455

    Purpose

    Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

    Depositor

    Brad Zuchero

    Article

    Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

    Insert

    DeAct-SpvB (spvB Salmonella enterica)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Truncation spanning SpvB amino acids 375-591 (mon…

    Promoter

    TRE3G

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available Since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Flag Intersectin Long (human)

    Plasmid

    #47393

    Depositor

    Peter McPherson

    Article

    Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

    Insert

    intersectin long (ITSN1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. 5HT6-YFP-ThymosinBeta4(KK18,19EE)

    Plasmid

    #96807

    Purpose

    non-actin-binding control for 5HT6-YFP-ThymosinBeta4(WT)

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    KK18,19EE prevents G-actin binding

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-Rab7a-7

    Plasmid

    #56443

    Purpose

    Localization: Ras GTPase, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Article

    Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N117S in NM_004637.5

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pODN-mR2-ACTB

    Plasmid

    #183868

    Purpose

    Repair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB homology arms with mRuby2-linker (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    mRuby2-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid

    #174631

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
    

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. plko-shmfn2-mfn2-Rescue

    Plasmid

    #160655

    Purpose

    "Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mfn2 (MFN2 Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR201-Eps8L2

    Plasmid

    #196526

    Purpose

    Entry vector for Gateway with Eps8L2

    Depositor

    Edgar Gomes

    Article

    Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

    Insert

    Eps8L2-FL (EPS8L2 Human)
    

    Expression

    Bacterial

    Promoter

    CMV promoter; T7 promoter

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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