We narrowed to 3,226 results for: GRI

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pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_816

Plasmid

#216098

Purpose

CRISPRa, EF1a-driven ALFA-dCas12a-VP64 (Cas only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

Cas12a [EnAs]

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1_35-345_eGFP

Plasmid

#208881

Purpose

Express eGFP-TLNRD1 lacking N and Cterm domains (TLNRD1 amino acids 35-345) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acid sequence 35-345

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mito-PDCD10-mScarlet-I

Plasmid

#208887

Purpose

Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM3 (PDCD10 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

PDCD10 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_5H_eGFP

Plasmid

#208861

Purpose

Express eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 5 helix bundle (TLNRD1 Human)

Expression

Mammalian

Mutation

Deleted amino acid 142-273

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p10xUAS-WHaloCaMP1a-EGFP

Plasmid

#205314

Purpose

Expression of WHaloCaMP1a-EGFP in Drosophila using GAL4 driver

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Expression

Insect

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP-eNOSpep-EGFP

Plasmid

#205306

Purpose

Expression of WHaloCaMP-eNOSpep-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP-eNOSpep-EGFP

Expression

Bacterial

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_B1ChR2_TS_mScarlet_ER

Plasmid

#195191

Purpose

Highly K+ selective channelrhodopsin

Depositor

Peter Hegemann, Johannes Vierock

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Bilabrum sp. K+ selective channelrhodopsin 2

Tags

ER-export sequence, Kir2.1 trafficking motif, and…

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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TERTp124_Gluc_CCR5

Plasmid

#192271

Purpose

Donor template for insertion of hTERT promoter (-124 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-124 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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TERTp146_Gluc_CCR5

Plasmid

#192272

Purpose

Donor template for insertion of hTERT promoter (-146 C to T) driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Mutation

-146 C to T

Promoter

TERT promoter driving expression of gaussia

Available Since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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Full-length P229S talin1

Plasmid

#182993

Purpose

express FL variant P229S talin1 in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin1 P229S (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

P229S

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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mini-talin P229S (headR13DD P229S)

Plasmid

#182995

Purpose

expression of minitalin P229S in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

Talin 1 (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

P229S

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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talin1-head P229S

Plasmid

#182997

Purpose

expression of talin1 head P229S in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

talin1 head P229S (Tln1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

P229S

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Kindlin

Plasmid

#186136

Purpose

For expression of recombinant Snap-labeled kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VP16

Plasmid

#183935

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VP16 (UL48 Synthetic)

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pB80-SSPB(micro)-mVenus-ppKin14VIb(861-1321)

Plasmid

#174641

Purpose

Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

SSPB(micro)-mVenus-ppKin14VIb(861-1321)

Tags

SSPB(micro)-Venus

Expression

Mammalian

Mutation

SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

Promoter

Chicken beta-actin

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Ovol1

Plasmid

#172871

Purpose

Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Ovol1 (Ovol1 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG F405G

Plasmid

#169956

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F405G

Promoter

CMV

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 CARD8 FIIND L370G

Plasmid

#169970

Purpose

pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

Depositor

Daniel Bachovchin

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Gateway cloning vector

Mutation

L370G

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-EGFP-TPD53

Plasmid

#172461

Purpose

Expression of untagged Tumor Protein D52 like 1 (TPD53/TPD52L1) and GFP

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD53 (TPD52L1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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