We narrowed to 29,570 results for: des.2

1. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

   Plasmid

   #169596

   Purpose

   Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2

   Plasmid

   #128760

   Purpose

   Expresses PARN gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PARN sgRNA #2 (PARN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

   Plasmid

   #128757

   Purpose

   Expresses PAPD4 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   PAPD4 sgRNA #1 (TENT2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZCS36 (Phsp16.41::Cas9(dpiRNA)::tbb-2 ‘3UTR)

   Plasmid

   #193048

   Purpose

   Heatshock expressed Cas9

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   hsp16.41p::Cas9(dpiRNA)::tbb-2 ‘3UTR
   

   Use

   CRISPR

   Expression

   Worm

   Promoter

   hsp16.41p

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3341 pcDNA3 Bcl-2 T69A S70A S87A

   Plasmid

   #13340

   Depositor

   Stanley Korsmeyer

   Article

   Yamamoto et al Mol Cell Biol 1999 Dec;19(12):8469-78.

   Insert

   Bcl-2 (BCL2 Human)
   

   Expression

   Mammalian

   Mutation

   changed Threonine 69 to Alanine; changed Serine 7…

   Available Since

   Nov. 9, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pLV-SARS-CoV-2 SL1 alone-Luciferase

   Plasmid

   #191482

   Purpose

   Luciferase reporter for SARS-CoV-2 5' UTR SL1 alone

   Depositor

   Hao Wu

   Article

   Shi et al bioRxiv. 2020 Sep 18. doi: 10.1101/2020.

   Insert

   SARS-CoV-2 5' UTR SL1 alone
   

   Use

   Lentiviral and Luciferase

   Mutation

   Contains only the stem-loop1 region of SARS-CoV-2…

   Available Since

   Nov. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATF4 17: 5’ATF4.uORF1stopm1&2.GFP

   Plasmid

   #21863

   Depositor

   David Ron

   Article

   Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

   Insert

   ATF4 (Atf4 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Stop codons between the colinear uORF1 and uORF2 …

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-2)

   Plasmid

   #159657

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Promoter

   Ef1a

   Available Since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106353

   Purpose

   CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   D4Z4 gRNA-2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABEhygro-Flag-ZO-2-1st-PDZ-mut

    Plasmid

    #27474

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K38E, F44H, I46E and V48E

    Available Since

    March 21, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pL6puro-pSV40(2)-pmRevErbA-Venus-NLS-Pest

    Plasmid

    #240113

    Purpose

    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter.

    Depositor

    Achim Kramer

    Article

    Gabriel et al bioRxiv 2025.06.30.661926

    Insert

    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    

    Use

    Lentiviral

    Mutation

    added SV40-Enhancer+Ori

    Available Since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2

    Plasmid

    #128763

    Purpose

    Expresses DIS3L2 gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    DIS3L2 sgRNA #2 (DIS3L2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p.UTA.2.0-GL-2-3 perfect copies

    Plasmid

    #82441

    Purpose

    Dual Fluorescent endogenous miRNA sensor Control insert. Three Perfect copies of complementary region of a siRNA control targeting firefly luciferase encoded by the pGL2

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    Luciferase
    

    Mutation

    The complementary region for GL2 siRNA control wa…

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. LB1554 (Bcl-2 promoter with MHS1234, Cdx mut)

    Plasmid

    #15384

    Depositor

    Linda Boxer

    Article

    Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Bcl-2 full promoter with IgH full enhancer MHS123…

    Available Since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pLV-SARS-CoV-2 5'UTR-Luciferase

    Plasmid

    #191480

    Purpose

    Luciferase reporter for SARS-CoV-2 5' UTR

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR
    

    Use

    Lentiviral and Luciferase

    Mutation

    contains SARS-CoV-2 5' UTR

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-5X-3-SARS-CoV-2-3CL

    Plasmid

    #168457

    Purpose

    Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

    Depositor

    David Ho, Sho Iketani, Alejandro Chavez

    Article

    Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

    Insert

    SARS-CoV-2 3CL protease (ORF1ab Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

    Plasmid

    #112837

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SH2 + SH3 (C-term) domains of Grb2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

    Plasmid

    #220797

    Purpose

    shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

    Insert

    EGFP
    

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiFC-HA-hCaspase 2 FL (C303S)-VC155

    Plasmid

    #49257

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    C303S

    Promoter

    CMV

    Available Since

    March 14, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLV-mScarlet-SARS-CoV-2-5'UTRΔSL1

    Plasmid

    #191484

    Purpose

    Lentiviral expression of SARS-CoV-2 5' UTR ΔSL1

    Depositor

    Hao Wu

    Article

    Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

    Insert

    SARS-CoV-2 5' UTR ΔSL1 (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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