We narrowed to 5,570 results for: donor

1. pDONR223_NKX2-1_DelCTerm_1-223AA

   Plasmid

   #221476

   Purpose

   Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Mutation

   Deletion of 224-371AA C-terminal domain

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_NKX2-1_DelNTerm_160-371AA

   Plasmid

   #221475

   Purpose

   Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Gateway donor vector

   Mutation

   Deletion of 1-159AA N-terminal domain

   Available Since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rif1-Halo HRD

   Plasmid

   #207081

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   None

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. RNF169-Halo HRD

   Plasmid

   #207083

   Purpose

   Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   May 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAVS1 SNAP-Sec61B

   Plasmid

   #207580

   Purpose

   Homologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   TET inducible SNAPTag-Sec61B
   

   Tags

   SNAPTAG

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SNAP-LC3 HRD

   Plasmid

   #207545

   Purpose

   Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
   

   Tags

   SNAPtag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Halo-ATG5 HRD

   Plasmid

   #207542

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG5 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag flanked by human ATG5 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Halo-LC3B HRD

   Plasmid

   #207539

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   Endogenous

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221-W279A-short-UBASH3A-Ctag

   Plasmid

   #195402

   Purpose

   Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Use

   Gateway donor vector

   Mutation

   W279A

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221-K202R-short-UBASH3A-Ctag

    Plasmid

    #195399

    Purpose

    Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Gateway donor vector

    Mutation

    K202R

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORANGE Tubb3-2xGFP KI

    Plasmid

    #183443

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 2xGFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOC4 Kcnn2-smFP-myc KI

    Plasmid

    #183438

    Purpose

    FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP myc donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOC2 GFP-Mpp2 KI

    Plasmid

    #183434

    Purpose

    CreOFF knock-in for GFP-MPP2 (amino acid position: A4)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOC5 smFP-HA-Cacna1e KI

    Plasmid

    #183441

    Purpose

    FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP HA donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOC2 Gria1-Halo KI

    Plasmid

    #183433

    Purpose

    CreOFF knock-in for GluA1-Halo (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORANGE Tubb3-3xGFP KI

    Plasmid

    #183444

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 3xGFP donor
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOC2 GFP-Actb KI

    Plasmid

    #183429

    Purpose

    CreOFF knock-in for GFP-beta-actin (amino acid position: before start codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOC2 Gria1-GFP KI

    Plasmid

    #183430

    Purpose

    CreOFF knock-in for GluA1-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and GFP donor
    

    Use

    CRISPR

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX552-rScn2a-3xV5-Syn-miRFP670

    Plasmid

    #182566

    Purpose

    For rat Nav1.2 knockin with 3xV5 at the extracellular loop

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    rat Scn2a gRNA and 3xV5 donor DNA
    miRFP670
    

    Use

    AAV and CRISPR

    Promoter

    Syn and U6

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRecomb-loxP-mCherry-4Gcloningsite-lox2272-66

    Plasmid

    #168787

    Purpose

    Donor vector for recombinase mediated integration in filamentous fungi. Is a shuttle vector for optional in vivo cloning in yeast, and contains four inducible promoters or sites for whole BGC cloning.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology; Expression in fila…

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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