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We narrowed to 4,840 results for: donor

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Showing: 2061 - 2080 of 4840 results

  10. pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

    Plasmid
    #197066
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP
    Depositor
    Julia Mahamid
    Publication
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Encapsulin
    Use
    CRISPR
    Tags
    FKBP and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197066-plasmid-map-sequence-id-402604

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  12. pAC148-pCR8-dCas9VP96

    Plasmid
    #48220
    Purpose
    dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP96 activation domain
    Use
    CRISPR and Gateway Cloning
    Tags
    HA Tag and VP96
    Mutation
    D10A;H840A
    Available since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48220-plasmid-map-sequence-id-69505

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  14. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid
    #227332
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227332-plasmid-map-sequence-id-452132

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  15. qTAG-C-mStayGold-Puro-PXN

    Plasmid
    #227317
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227317-plasmid-map-sequence-id-452087

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  16. qTAG-C-mStayGold-Puro-CANX

    Plasmid
    #227281
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CANX Homology Arms flanking a mStayGold-2A-Puro Cassette (CANX Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227281-plasmid-map-sequence-id-452050

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  17. qTAG-AAVS1-PGK-Zeo

    Plasmid
    #227271
    Purpose
    Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Expression
    Mammalian
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227271-plasmid-map-sequence-id-452027

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  19. qTAG-C-miRFP670nano3-Blast-H3C2

    Plasmid
    #207784
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207784-plasmid-map-sequence-id-416184

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  20. pCL026 AAVS1-puroR-9xTet0-pPGK-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

    Plasmid
    #196545
    Purpose
    Donor plasmid for integrating PGK promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus
    Depositor
    Lacra Bintu
    Publication
    DelRosso et al Nature. 2023 Apr;616(7956):365-372. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Surface marker and Citrine reporter
    Use
    Synthetic Biology
    Expression
    Mammalian
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    196545-plasmid-map-sequence-id-384575

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Showing: 2061 - 2080 of 4840 results