We narrowed to 5,844 results for: pcas

1. pAAV-pCALM1-EYFP-WPRE-bGHpA

   Plasmid

   #213971

   Purpose

   AAV expression of EYFP from pCALM1 promoter

   Depositor

   Zhonghua Lu

   Article

   Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

   Insert

   pCALM1
   

   Use

   AAV

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SpCas9(H840A)-T2A-EGFP

   Plasmid

   #221231

   Purpose

   Express nCas9 nickase (H840A) with GFP reporter

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   SpCas9(H840A)-T2A-EGFP
   

   Expression

   Mammalian

   Mutation

   H840A

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pCAG-FLEX-EGFP-WPRE

   Plasmid

   #51502

   Purpose

   Can be used to generate AAV virus that will express EGFP in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-HiFi SpCas9-NLS-6xHis

   Plasmid

   #207376

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R691A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

   Plasmid

   #103849

   Purpose

   dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Promoter

   CMV

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0043-PspCas13b-gRNA non target

   Plasmid

   #223698

   Purpose

   gRNA control for dPspCas13b-FTO

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   gRNA target sequence for luciferase control
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX335-U6-Chimeric_BB-CBh-hSpCas9n(D10A)

   Plasmid

   #42335

   Purpose

   A human codon-optimized SpCas9 nickase and chimeric guide RNA expression plasmid.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Has Service

   Cloning Grade DNA

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

   Plasmid

   #190111

   Purpose

   AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   AAV

   Tags

   bpNLS

   Mutation

   H840A

   Promoter

   EF1a

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAG001 His6-TwinStrep-SUMO-AapCas12b

    Plasmid

    #153162

    Purpose

    Bacterial expression plasmid for AapCas12b

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

    Insert

    AapCas12b
    

    Expression

    Bacterial

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC0046-EF1a-PspCas13b-NES-HIV

    Plasmid

    #103862

    Purpose

    Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCAG ERT2-Cre-ERT2 lox

    Plasmid

    #183447

    Purpose

    CreOFF tamoxifen-inducible Cre

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    ERT2-Cre-ERT2
    

    Expression

    Mammalian

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

    Plasmid

    #132405

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA14-pHR-SFFV-GFP-RPA43

    Plasmid

    #199445

    Purpose

    Expression of GFP-RPA43 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    RPA43 (POLR1F Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. CROPseq-EFS-HypaSpCas9-P2A-EGFP

    Plasmid

    #106964

    Purpose

    single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

    Depositor

    Alex Hewitt

    Insert

    HypaSpCas9
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG PSAM4 GlyR IRES EGFP

    Plasmid

    #119739

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Synthetic, Human)
    

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    CAG

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

    Plasmid

    #139994

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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