We narrowed to 22,473 results for: SCR

1. pZF-ADD

   Plasmid

   #127468

   Purpose

   coding sequence for synthetic zinc finger ZF-ADD

   Depositor

   Sebastian Maerkl

   Article

   Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

   Insert

   ZF-ADD synthetic transcription factor
   

   Use

   Cell-free transcription–translation

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZF-ADB

   Plasmid

   #127467

   Purpose

   coding sequence for synthetic zinc finger ZF-ADB

   Depositor

   Sebastian Maerkl

   Article

   Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

   Insert

   ZF-ADB synthetic transcription factor
   

   Use

   Cell-free transcription–translation

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HRE-luciferase

   Plasmid

   #26731

   Purpose

   Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

   Depositor

   Navdeep Chandel

   Article

   Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

   Insert

   Hypoxia Responsive Elements (3)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-ATR WT

   Plasmid

   #31611

   Depositor

   Aziz Sancar

   Article

   Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. mt POLRMT

   Plasmid

   #205059

   Purpose

   POLRMT protein expression

   Depositor

   Smita Patel

   Article

   Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

   Insert

   POLRMT (POLRMT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized

   Promoter

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207180

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. KRAB-dSpCas9

   Plasmid

   #205248

   Purpose

   In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-N1-delta30-TFEB

   Plasmid

   #44445

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-30; H32D

   Promoter

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC009.106

   Plasmid

   #171142

   Purpose

   Expression of J106 scRNA on pBBR1-KmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J106 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMH-EF1-GFP

    Plasmid

    #133018

    Purpose

    (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Dec. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLuc-CDS

    Plasmid

    #42100

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNG1299

    Plasmid

    #149709

    Purpose

    Template for Malachite Green Aptamer (MGA) transcription assay

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    Triple Promoter - 12X MG Aptamer Array - Terminator construct
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWPT-PDX1

    Plasmid

    #12256

    Depositor

    Didier Trono

    Article

    Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

    Insert

    Pdx1 (PDX1 Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLV-REPORT (PGK) NanoLuc

    Plasmid

    #208576

    Purpose

    Empty NanoLuc Lentiviral reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    NanoLuciferase
    d2eGFP
    Empty response element
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    3x SV40 NLS and PEST

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and miniP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 Flag Runx1 FL (P#1790)

    Plasmid

    #14585

    Depositor

    Matija Peterlin

    Article

    Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pegfp-N1-TFEB-S3A,R4A

    Plasmid

    #44446

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFEB (TFEB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed both Serine 3 and Arginine 4 to Alanine

    Promoter

    CMV

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NLSx3-CRY2PHR-p65

    Plasmid

    #64124

    Purpose

    Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    CRY2PHR-p65
    

    Use

    Tags

    His, NLSx3, and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PE_CO

    Plasmid

    #190335

    Purpose

    Prime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cells

    Depositor

    Rasmus Bak

    Article

    Gao et al Mol Ther. 2022 Jul 8. pii: S1525-0016(22

    Insert

    PE2 with codon-optimized reverse transcriptase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-REPORT(PGK)-12xHRE

    Plasmid

    #172334

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-TET2Puro-gal4DBD-miniVPR-HA

    Plasmid

    #207169

    Purpose

    Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-miniVPR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEF-TAZ-N-Flag

    Plasmid

    #19025

    Depositor

    Michael Yaffe

    Article

    Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

    Insert

    TAZ (Wwtr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Vector has no SV40 origin

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

22. pMH-CMV-GFP

    Plasmid

    #133017

    Purpose

    (Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under CMV promoter.

    Depositor

    Michael Huen

    Article

    Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pQCXIN-EF1a-mNeonGreen-P2A-Cas9

    Plasmid

    #189807

    Purpose

    Retroviral delivery of Cas9

    Depositor

    Ellen Rothenberg

    Article

    Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLuc-3'UTR

    Plasmid

    #42094

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    ZEB1 3UTR (ZEB1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

    Plasmid

    #203911

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C2]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

    Plasmid

    #203910

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C1]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR1A_no_ccDB_mUty

    Plasmid

    #158570

    Purpose

    V5-tagged sequence of mUty cloned into entry vector for recombination

    Depositor

    Christian Deschepper

    Article

    Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

    Insert

    Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEBG-hBEN

    Plasmid

    #22160

    Depositor

    Ananda Roy

    Article

    Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEBB-GFP-hBEN

    Plasmid

    #22157

    Depositor

    Ananda Roy

    Article

    Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPPC017.109

    Plasmid

    #171145

    Purpose

    Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J109 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV-REPORT(EF1a no GFP) mnuctdTomato

    Plasmid

    #172342

    Purpose

    mnuctdTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPPC020.306

    Plasmid

    #171148

    Purpose

    Expression of J3-BBa_J23117-mRFP and J306 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J306 scRNA
    J3-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J3-BBa_J23117

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pZF-BCB-GCN

    Plasmid

    #127476

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pZF-BCB-PDZ

    Plasmid

    #127478

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-PDZ

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    

    Use

    Cell-free transcription–translation

    Tags

    alpha 1-syntrophin PDZ domain

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZF-ADD-L

    Plasmid

    #127477

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-L

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    

    Use

    Cell-free transcription–translation

    Tags

    PDZ cognate peptide ligand (VKESLV)

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZF-ADD-GCN

    Plasmid

    #127475

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRCA360 - pBA904 Puro-T2A-GFP

    Plasmid

    #238164

    Purpose

    Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    Non-targeting sgRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pLV-tet2puro-gal4DBD-HIFCAD

    Plasmid

    #207173

    Purpose

    Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    Gal4DBD-HIF1a-CAD
    

    Use

    Lentiviral; Dox inducible

    Tags

    2x Myc

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. eSpCas9-EF

    Plasmid

    #177267

    Purpose

    CRISPR vector

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPPC016.109

    Plasmid

    #171144

    Purpose

    Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J109 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge