We narrowed to 4,135 results for: p2a

1. pK168.AAV-EF1α-DIO-tTA-P2A-RFP-WPRE (Supernova)

   Plasmid

   #85039

   Purpose

   For AAV-TRE-Cre based Supernova-RFP, pK168 should be used with pK170.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   RFP-P2A-tTA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82706

   Purpose

   AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   U6/TO Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

   Plasmid

   #223959

   Purpose

   Dual fluorescent reporter for histone H2B and for APC/C activity

   Depositor

   Hee Won Yang

   Article

   Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

   Insert

   H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

   Plasmid

   #188774

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

   Plasmid

   #223965

   Purpose

   Dual fluorescent reporter for histone H2B and for Cdt1

   Depositor

   Hee Won Yang

   Article

   Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

   Insert

   H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE

   Plasmid

   #234895

   Purpose

   CAG-driven, Flp-dependent ChRmine for optogenetic excitation of neurons with an oScarlet tag

   Depositor

   Silvia Arber

   Article

   Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

   Insert

   FRT(F3F5)-ChRmine3.3-p2A-oScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

   Plasmid

   #125430

   Purpose

   CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK and U6

   Available since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

   Plasmid

   #169337

   Purpose

   Expression of the LT-Fucci(SCA) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Fucci(SCA) (GMNN, CDT1 Human)
   

   Use

   Tags

   HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

   Plasmid

   #208291

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-P2A-EGFP

   Expression

   Mammalian

   Mutation

   ABE8e mutations and V106W in TadA and nSpRY(D10A/…

   Promoter

   CMV and T7

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

    Plasmid

    #208292

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA inlaid into nSpCas9(D10A)

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

    Plasmid

    #118169

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2

    Plasmid

    #118170

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide3

    Plasmid

    #118171

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS101 pHAGE2 CMVtetO2 TagBFP-P2A-ALFA-22xGS-SUMOEu-MCS

    Plasmid

    #199354

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally BFP-P2A-ALFA-SUMOEu-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP-P2A-ALFA-SUMOEu

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRCA918 - TFORF Empty Vector Puro-P2A-BFP (pRCA892 backbone)

    Plasmid

    #238183

    Purpose

    Lentiviral empty vector

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pInducer(p20) x mKate2-NLS-P2A-HRas(N17)_ires-puro

    Plasmid

    #167832

    Purpose

    Inducible expression of dominant negative HRas(N17) and P2A-coupled reporter mKate2-NLS

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    HRAS(N17) (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    S17N

    Promoter

    Ubc, TRE

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

    Plasmid

    #232800

    Purpose

    Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

    Depositor

    Chunliang Li

    Article

    Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

    Insert

    OsTIR1 F74G EGFP-AID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

    Plasmid

    #219781

    Purpose

    CaST-P2A for HEK cell expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

    Plasmid

    #162566

    Purpose

    Used for the dual color labeling of cPLA2 and LaminB1 expression.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

    Inserts

    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    Lamin B1 (lmnb1 Zebrafish)
    

    Use

    Tags

    EGFP, P2A, and mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SV40

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge