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pRK5-Gqi9

Plasmid

#125711

Purpose

Eukaryotic expression of the chimeric G protein Gqi9

Depositor

Kai Johnsson

Article

Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

Insert

chimericG protein Gqi9

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9

Plasmid

#137079

Purpose

Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pDD372

Plasmid

#91824

Purpose

Codon-optimized GFP donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

GFP-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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CENP-B mCherry

Plasmid

#45219

Depositor

Michael Lampson

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

CENP-B mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

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pBabe 12S E1A

Plasmid

#18742

Depositor

Scott Lowe

Article

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pJTM-T7-mNeongreen-6xHis

Plasmid

#210868

Purpose

Plasmid used for expressing his--tagged mNeongreen for protein purification.

Depositor

Richard Murray

Article

Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

Insert

mNeongreen-6xHistag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pProm1_BCD1-GFP

Plasmid

#80649

Purpose

Promoter1 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Mutation

NONE

Promoter

constitutive promoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pPSU20

Plasmid

#173829

Purpose

Expresses 20 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACCBPv3

Expression

Bacterial

Promoter

T7

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP18

Plasmid

#80672

Purpose

Encodes the synthetic coiled coil peptide SYNZIP18

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP18

Use

Entry vector

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDB021CH(+)

Plasmid

#99209

Purpose

Accessory plasmid for PACE of PylRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII, luxAB; ProK pylT

Expression

Bacterial

Promoter

PT7 and PproK

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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