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pOBD2 Pab1 E181R (123-204)

Plasmid

#196835

Purpose

Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain

Depositor

Stan Fields

Article

Melamed et al PLoS Genet. 2015 Feb 11;11(2):e1004918.

Insert

Pab1 (PAB1 Budding Yeast)

Tags

Gal4

Expression

Yeast

Mutation

123-204 amino acids & E181R

Promoter

ADH

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRBx2

Plasmid

#193569

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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PH-mCherry-FRBx2

Plasmid

#193570

Purpose

Expresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and mCherry

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_FGF21

Plasmid

#182406

Purpose

Bacterial expression of fgf21

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

fgf21 (FGF21 Human)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-DARPinOFF7

Plasmid

#182414

Purpose

Bacterial expression of mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-DARPinOFF7

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

Plasmid

#182393

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ
CydAB-mNeongreen

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mTq2-antiFRET-P2A-RCaMP1h

Plasmid

#191467

Purpose

Compare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

RCaMP1h

Tags

His-mTurquoise2-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-2X(CMV-eBFP2-3XNLS-pA)

Plasmid

#195867

Purpose

to express nuclear localized eBFP2

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

eBFP2

Use

AAV

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-EGFP

Plasmid

#191458

Purpose

Compare the intensity of EGFP to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

EGFP

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y

Plasmid

#191459

Purpose

Compare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent protein

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

mTurquoise2_T203Y

Tags

His-mScarletI-antiFRETlinker-P2A

Expression

Bacterial and Mammalian

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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