We narrowed to 8,166 results for: gne

1. pST_PvLEA4_repeats_k3_11 (pBS0761)

   Plasmid

   #185243

   Purpose

   For the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_11. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   PvLEA4_repeats_k3_11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pST_DrHD_dom3_k5_0 (pBS0758)

   Plasmid

   #185242

   Purpose

   For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k5_0. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DrHD_dom3_k5_0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pST_DrHD_dom3_k3_7 (pBS0755)

   Plasmid

   #185241

   Purpose

   For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_7. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DrHD_dom3_k3_7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pST_DrHD_dom3_k3_1 (pBS0754)

   Plasmid

   #185240

   Purpose

   For the mammalian expression of the Deinococcus radiodurans protein DrHD_dom3_k3_1. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   DrHD_dom3_k3_1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pST_CI016_HUMAN (pBS0747)

   Plasmid

   #185238

   Purpose

   For the mammalian expression of the human protein CI016_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   CI016_HUMAN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST_AdAPOA4-44 (pBS0745)

   Plasmid

   #185236

   Purpose

   For the mammalian expression of the Chinese giant salamander protein AdAPOA4-44. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   AdAPOA4-44
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KTK_045

   Plasmid

   #180543

   Purpose

   Entry vector containing RBS 30,000 a.u. designed strength

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   sRBS 30,000 a.u. designed strength
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. KTK_046

   Plasmid

   #180544

   Purpose

   Entry vector containing RBS 250 a.u. designed strength

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   sRBS 250 a.u. designed strength
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330_ACTB-e1 sgRNA / hSpCas9

   Plasmid

   #172830

   Purpose

   Mammalian expression of a sgRNA targeting the exon 2 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX330_ACTB-e2 sgRNA / hSpCas9

    Plasmid

    #172831

    Purpose

    Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330_Camk2a sgRNA / hSpCas9

    Plasmid

    #172839

    Purpose

    Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330_ACTB-i5 sgRNA / hSpCas9

    Plasmid

    #172829

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX330_ACTB-i3 sgRNA / hSpCas9

    Plasmid

    #172827

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX330_ACTB-i2 sgRNA / hSpCas9

    Plasmid

    #172826

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-GFP-EBA175-Flag(P1424G)

    Plasmid

    #155128

    Purpose

    Expresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    P1424G; insert codes for EBA175 residues 1284 to …

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-HA-PfROM4 (H578A)

    Plasmid

    #154858

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant H578A (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Histidine 578 changed to Alanine

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-HA-PfROM4 (G470A/G471N)

    Plasmid

    #154859

    Purpose

    Expresses N-terminally HA-tagged PfROM4 variant G470A/G471N (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM4 (PF3D7_0506900 Synthetic)
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Glycine 470 changed to Alanine and Glycine 471 ch…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-HA-PfROM1 (A144G/N145G)

    Plasmid

    #154873

    Purpose

    Expresses N-terminally HA-tagged PfROM1 variant A144G/N145G (codon optimized for mammalian expression) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfROM1 (PF3D7_0506900 Synthetic)
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    Alanine 144 changed to Glycine and Asparagine 145…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-Basic-ZC3H12D_PromoterWT

    Plasmid

    #153065

    Purpose

    ZC3H12D promoter region cloned upstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    ZC3H12D promoter region (ZC3H12D Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UAS-u(XXL)Cas9

    Plasmid

    #127387

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XXL)-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge