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We narrowed to 363 results for: gne

Showing: 1 - 20 of 363 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...whenever the magnet approached the sample (by less than 1%) and recovered after the magnet was removed ...in response to a ~25 mT magnet. Gray regions indicate times when the magnetic field is on. Image reproduced...in response to a ~10 mT magnet. Gray regions indicate times when the magnetic field is on. Image reproduced...fluorescent proteins respond to magnetic fields?” and “What are magnetoresponsive fluorescent proteins good ...Robinson, J. T. (2019). Magnetic Entropy as a Proposed Gating Mechanism for Magnetogenetic Ion Channels. Biophysical...Chemical amplification of magnetic field effects relevant to avian magnetoreception. Nature Chemistry, 8(4...fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024). They deposited...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...Zhao and colleagues, we now have just the tool…designer PUF proteins! A newly available PUF Assembly Kit...In a single step, researchers can now assemble designer PUF domains for RNA specificity engineering. “...translational repressor. In principle, however, designer PUF proteins can be fused to other components ...Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific post-transcriptional...

  4. TALEN Plasmids and Kits

    Type
    Collection
    ... pre-assembled trimer and tetramer RVD domains designed for rapid TALEN assembly. Kiran Musunuru and Chad...plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN are designed as destination vectors for the Voytas lab Golden...truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in vitro synthesis of TALEN mRNAs. Both 5’...BB Tom Ellis Plasmids pTAL5-BB and pTAL6-BB are designed as alternative destination vectors for the Golden...-RR are next generation TALEN backbone vectors designed for use with the Voytas Golden Gate TALEN Kit ...-T7-TALEN(Sangamo)-Destination constructs were designed for optimal mammalian expression of Voytas Golden...mammalian cell-based validation assay that are designed for use with the Golden Gate TALEN and TAL Effector...

  5. CRISPR Plasmids and Resources

    Type
    Collection
    ...Bacteria CRISPR plasmids designed for use in bacteria. C. elegans CRISPR plasmids designed for use in C. elegans... CRISPR plasmids designed for use in Drosophila . Mammalian CRISPR plasmids designed for use in mammalian...Parasites CRISPR plasmids designed for use in parasites. Plant CRISPR plasmids designed for use in plants. Yeast...Yeast CRISPR plasmids designed for use in yeast. Xenopus CRISPR plasmids designed for use in Xenopus . .... Zebrafish CRISPR plasmids designed for use in zebrafish....

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...vector before the sgRNA scaffold. The oligos are designed based on the target site sequence (20bp) and need...be cloned into the CRISPR array. The oligos are designed based on the target site sequence (30bp) and need...using SAM: Addgene plasmids #61422 - #61424 were designed for transient transfection : 61422 : Expresses...vector before the sgRNA scaffold. The oligos are designed based on the target site sequence (21-22bp) and...ligation of annealed and phosphorylated oligos designed based on the target site sequence (20bp). CRISPR-Cas9...neuronal-specific gene NeuN. As a control they designed an sgRNA targeting LacZ, which is not present ...ligation of annealed and phosphorylated oligos designed based on the target site sequence (20bp). #60230...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are sensitive... or organisms for long-term imaging and can be designed to specifically localize to cellular compartments...17404-9. Ca2+ indicators based on computationally redesigned calmodulin-peptide pairs. Chem Biol. 2006 May...Labeling of active neural circuits in vivo with designed calcium integrators. Science. 2015 Feb 13;347(...reticulum. Biophys Rep (2019) 5, 31–42 Pingyong Xu Magnesium Fluorescent Mg2+ sensor (MARIO) A Transient Rise.... 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean/Citrine...Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors. Science. 2018 Jun...

  8. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was magnetically...revealed that Magneto 2.0 allows for magnetic remote control of neuronal activation. Find Magneto 2.0 plasmids.... Methods 2016. PubMed PMID: 27798611 Magneto 2.0 - A magnetic remote control for the nervous system ...waves or allow for magnetogenetic control, but these actuators were not optimally designed for neuronal systems... 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway...Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4...Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells, targets...

  9. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...plasmids have been designed for use in Xenopus. Cut Fully functional Cas9 enzymes designed to introduce a ...CRISPR plasmids that have been designed for use in Xenopus. CRISPR...

  10. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Blog Posts The table below lists gRNA plasmids designed to target various genes or genomic regions. It...pyogenes , S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...unpublished plasmids containing gRNA sequences designed to be used with any CRISPR nuclease and function...

  11. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...collected by centrifugation (agarose beads) or a magnet (magnetic beads), providing a solid support while unbound...surface of magnetic beads, and are therefore completely accessible. The yields from magnetic beads are ... The beads are collected by centrifugation or a magnet. 4) Unbound proteins are washed away. 5) The target...protein is immobilized, or tethered, on agarose or magnetic beads and then incubated with a protein mix. During...often called the capture antibody, to agarose or magnetic beads in a way that also allows the antibody to...recombinant Protein A/G are conjugated to agarose or magnetic beads and allowed to bind to the antibody being...antibodies can be directly conjugated to agarose or magnetic beads with commercially available chemical agents...

  12. Distribution to Industry

    Type
    Collection
    ...PDB-deposited), disulfide-free, mesophilic, and designed to be diverse in topology, size, and isoelectric... will send you a copy of the MTA, which must be signed by an authorized signatory at the recipient institution...the recipient institution. Once the MTA has been signed, it can be submitted to Addgene through your user...

  13. Plant Plasmids and Resources

    Type
    Collection
    ...contains many plasmid tools that were specifically designed for use in plants. These resources include tools...Caixia Gao. Cloning The following collections are designed for creating plant expression vectors: A modular...Silin Zhong. RNAi The following collections are designed for gene silencing in plants: microRNAs in Arabidopsis...

  14. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ..., you mix specially coated magnetic beads into your sample and use magnets to isolate the beads for the...components (i.e. buffers, columns, or beads) are designed to work together — no mixing and matching!   ... use one of two common approaches — columns or magnetic beads. Both approaches involve binding the plasmid...bacterial culture. Plasmid purification kits are designed to accommodate specific volume ranges of culture...column-based miniprep kit for example. These kits are designed to handle ~1–5 mL of liquid culture. Using a higher...have an appropriate amount of beads and a suitable magnet as you scale up. Table 1: Standard Prep Kit Capacities.... To help combat this, some manufacturers have designed eco-friendly DNA purification kits by reducing...

  15. Antibody Guide

    Type
    Collection
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...

  16. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...replacing it or disrupting it with an piece of DNA designed in the lab. Specially constructed plasmids can...locus with homologous sequence using a specially designed vector that contains sequence homology to the ... the process, we’ll walk through an experiment designed to knock out exon 2 of a given gene.   ... Figure 1: A knockout targeting vector designed to insert a resistance gene. The vector contains a neomycin... Figure 2: A knockout targeting vector designed to insert and later remove a resistance gene. ...Figure 3: A knockout/knock-in targeting vector designed to insert GFP into a given locus.   Conditional...

  17. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...years of distributing his Designer Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids ... a rational protein engineering approach, they designed Opto-RTKs, which activate signaling cascades on...RNAi transgenic mice The Jackson-Grusby lab has designed two vectors for use in conditional and reversible...needed to insert new genes into bacteria. They designed the pOSIP (one-step integration plasmid) series...are you waiting for? Start building up your own designer bacteria. St-Pierre et al., ACS Synth Biol 2013...Check out pET-28b-Cas9-His from Alex Schier's lab, designed for expression and purification of Cas9 protein...insect and mammalian cells. These vectors were designed for parallel testing and screening of constructs...

  18. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...often used in real time PCR. These assays can be designed to detect either a donor sequence integrated through...the target locus, as well as a reference probe designed to bind at an unedited site distal tothe predicted...double strand break, and a NHEJ/ “drop-off” probe designed to bind directly at the predicted double strand...on their mutation status. The drop-off probe is designed to perfectly complement the wild type sequence...gene-editing frequencies (GEF-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed...

  19. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...have pre-designed plasmids for these systems! Ion sensors One common biosensor class is designed to monitor...interest for many disciplines! Most of the sensors designed for calcium generate a fluorescent output, which...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...voltage, and tension all have specific sensors designed for them. For example, this class includes tension...

  20. How Addgene Creates Content

    Type
    Blog Post
    ...they might have as they go. The guide was then assigned to our science communication specialist, Rachel...Since our guides are heavily used, our graphic designer made most of the Antibody Guide figures. This ...communication between our content team and our graphic designer to make sure things are clear, accurate, and in...Reference image (left) provided to our graphic designer upon request that the science communicator "just...). Immediate and immense regret of the graphic designer not shown.   However, for our blog posts, ...
Showing: 1 - 20 of 363 results