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We narrowed to 290 results for: gne

Showing: 1 - 20 of 290 results

  1. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...whenever the magnet approached the sample (by less than 1%) and recovered after the magnet was removed ...in response to a ~25 mT magnet. Gray regions indicate times when the magnetic field is on. Image reproduced...in response to a ~10 mT magnet. Gray regions indicate times when the magnetic field is on. Image reproduced...fluorescent proteins respond to magnetic fields?” and “What are magnetoresponsive fluorescent proteins good ...Robinson, J. T. (2019). Magnetic Entropy as a Proposed Gating Mechanism for Magnetogenetic Ion Channels. Biophysical...Chemical amplification of magnetic field effects relevant to avian magnetoreception. Nature Chemistry, 8(4...fluorescent protein tool that responds strongly to magnetic fields (Frank Hayward et al., 2024). They deposited...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...Zhao and colleagues, we now have just the tool…designer PUF proteins! A newly available PUF Assembly Kit...In a single step, researchers can now assemble designer PUF domains for RNA specificity engineering. “...translational repressor. In principle, however, designer PUF proteins can be fused to other components ...Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific post-transcriptional...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...named it Magneto 2.0. Using Magneto2.0-p2A-mCherry constructs, the lab verified that Magneto2.0 was magnetically...revealed that Magneto 2.0 allows for magnetic remote control of neuronal activation. Find Magneto 2.0 plasmids.... Methods 2016. PubMed PMID: 27798611 Magneto 2.0 - A magnetic remote control for the nervous system ...waves or allow for magnetogenetic control, but these actuators were not optimally designed for neuronal systems... 74308 pcDNA3.0-Magneto2.0-p2A-mCherry Mammalian Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway...Gateway 74333 pCR8-Magneto2.0 Gateway 74307 pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4...Drosophila community as well. The pooled library, designed to be used with cultured Drosophila cells, targets...

  5. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...collected by centrifugation (agarose beads) or a magnet (magnetic beads), providing a solid support while unbound...surface of magnetic beads, and are therefore completely accessible. The yields from magnetic beads are ... The beads are collected by centrifugation or a magnet. 4) Unbound proteins are washed away. 5) The target...protein is immobilized, or tethered, on agarose or magnetic beads and then incubated with a protein mix. During...often called the capture antibody, to agarose or magnetic beads in a way that also allows the antibody to...recombinant Protein A/G are conjugated to agarose or magnetic beads and allowed to bind to the antibody being...antibodies can be directly conjugated to agarose or magnetic beads with commercially available chemical agents...

  6. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ..., you mix specially coated magnetic beads into your sample and use magnets to isolate the beads for the...components (i.e. buffers, columns, or beads) are designed to work together — no mixing and matching!   ... use one of two common approaches — columns or magnetic beads. Both approaches involve binding the plasmid...bacterial culture. Plasmid purification kits are designed to accommodate specific volume ranges of culture...column-based miniprep kit for example. These kits are designed to handle ~1–5 mL of liquid culture. Using a higher...have an appropriate amount of beads and a suitable magnet as you scale up. Table 1: Standard Prep Kit Capacities.... To help combat this, some manufacturers have designed eco-friendly DNA purification kits by reducing...

  7. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...replacing it or disrupting it with an piece of DNA designed in the lab. Specially constructed plasmids can...locus with homologous sequence using a specially designed vector that contains sequence homology to the ... the process, we’ll walk through an experiment designed to knock out exon 2 of a given gene.   ... Figure 1: A knockout targeting vector designed to insert a resistance gene. The vector contains a neomycin... Figure 2: A knockout targeting vector designed to insert and later remove a resistance gene. ...Figure 3: A knockout/knock-in targeting vector designed to insert GFP into a given locus.   Conditional...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...years of distributing his Designer Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids ... a rational protein engineering approach, they designed Opto-RTKs, which activate signaling cascades on...RNAi transgenic mice The Jackson-Grusby lab has designed two vectors for use in conditional and reversible...needed to insert new genes into bacteria. They designed the pOSIP (one-step integration plasmid) series...are you waiting for? Start building up your own designer bacteria. St-Pierre et al., ACS Synth Biol 2013...Check out pET-28b-Cas9-His from Alex Schier's lab, designed for expression and purification of Cas9 protein...insect and mammalian cells. These vectors were designed for parallel testing and screening of constructs...

  9. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...often used in real time PCR. These assays can be designed to detect either a donor sequence integrated through...the target locus, as well as a reference probe designed to bind at an unedited site distal tothe predicted...double strand break, and a NHEJ/ “drop-off” probe designed to bind directly at the predicted double strand...on their mutation status. The drop-off probe is designed to perfectly complement the wild type sequence...gene-editing frequencies (GEF-dPCR) mediated by designer nucleases. Nat Protoc 11, 598–615 (2016). PubMed...

  10. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...have pre-designed plasmids for these systems! Ion sensors One common biosensor class is designed to monitor...interest for many disciplines! Most of the sensors designed for calcium generate a fluorescent output, which...are sensors available for other ions including magnesium, potassium, and zinc. These ion sensors can be...voltage, and tension all have specific sensors designed for them. For example, this class includes tension...

  11. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...they might have as they go. The guide was then assigned to our science communication specialist, Rachel...Since our guides are heavily used, our graphic designer made most of the Antibody Guide figures. This ...communication between our content team and our graphic designer to make sure things are clear, accurate, and in...Reference image (left) provided to our graphic designer upon request that the science communicator "just...). Immediate and immense regret of the graphic designer not shown.   However, for our blog posts, ...

  12. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...DREADDs DREADDs DREADDS, or designer receptors exclusively activated by designer drugs, are G protein-coupled...Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chemical Neuroscience...Conditional expression and signaling of a specifically designed Gi-coupled receptor in transgenic mice. Nature...

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    Published
    Aug. 27, 2024, 1:15 p.m.
    ... the Funabiki Lab developed a technique called Magnetic Isolation and Concentration (MagIC)-cryo-EM that...enrichment and imaging of proteins captured on magnetic beads (Arimura et al., 2024). In MagIC-cryo-EM...proof-of-concept study) are captured on streptavidin-coated magnetic beads through an assembly of biotinylated SpyTag-SpyCatcher... identified by Topaz software (green circles), magnetic bead (*), and spacers are labeled; scale bar: ...2024) MagIC-Cryo-EM: Structural determination on magnetic beads for scarce macromolecules in heterogeneous...

  14. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ... the state of the RSM. If an attB-attP pair is aligned (sites facing the same direction) on a register...). Alternatively, if an attB-attP pair is anti-aligned (sites facing opposite directions) on a register...recognition sites from the register. If the RSM is designed in such a way that each possible order of inputs...were built with flanking primer binding sites (designed to have 20-30 nt length, ~60 oC annealing temperature...Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014...

  15. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...team was the first to develop and publish a human-designed protein that was not based on any existing protein...got closer and closer to achieving it. Once they designed the protein computationally, the researchers expressed... protein design is possible. Although Top7 was designed simply to be stable and novel, the principles ...Baker Lab and others. The introduction of de novo designed proteins also helped researchers understand more...

  16. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...construct, most often in special E. coli strains designed for protein expression. Cultures are then lysed...and Pichia pastoris pCri also includes plasmids designed for protein expression in systems other than E...other eukaryotes better than E. coli. Plasmids designed for these systems may include a signal peptide...difficult-to-purify membrane proteins. pCri vector pCri-13a, designed for use in B. subtilis, contains the MISTIC (membrane-integrating...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...cell events Three years ago, Brian Kuhlman’s lab designed photoswitchable dimers by fusing the SsrA peptide...gene is inserted. It is important that primers be designed to minimize the possibility of dimer formation...preposterous. Luckily, the Pierre Neveu laboratory designed a new iterative, chaining-based cloning method...incorporation of a variety of bio-orthogonal UAAs via a re-assigned stop codon (TAG). This polyspecificity enables...translation. This luciferase reporter system was also designed for a short screening time (<4 hours) in order...expression as anticipated. For instance, an aptamer designed to bind a transcriptional activator activated ...loci using validated gRNAs from Addgene and well designed homology directed repair templates (see the supplementary...

  18. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...range and high brightness. The Shu Lab from UCSF designed an improved apoptosis reporter, ZipGFP, which ...prevent binding, the β strands in FlipGFP were redesigned so that they are parallel to each other and thus...fluorogenic reporter toolkit, the Shu Lab also designed a red fluorogenic protease reporter based on superfolder...FlipCherry provides a proof of concept that the designed used in FlipGFP can be applied to other fluorescent...

  19. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ... launched earlier in the year, as well as our redesigned homepage and our new search tool, are the results...per resource category to expose as filters and designed the search engine to return only the filters that... bits of information for a given resource, and designed the search engine to weight search results differently...'s new search Read to learn more about why we redesigned our homepage and mascot ...

  20. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...customized to direct assembly of DNA fragments. When designed correctly, the recognition sites do not appear... scheme is as follows: the gene of interest is designed with Type IIS sites (such as BsaI or BbsI), that...eight amino acids, but Golden Gate assembly can be designed to be scarless. Golden Gate assembly is also less...create TAL effector nucleases. These plasmids were designed to utilize the BsaI and BsmBI Type IIS sites such...
Showing: 1 - 20 of 290 results