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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9 results for: gne

Showing: 1 - 9 of 9 results

  1. Chemogenetics Guide

    Type
    Guide
    ...less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), like RASSLs...Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chem Neurosci...1998). Controlling signaling with a specifically designed Gi-coupled receptor. Proc Natl Acad Sci USA. 95...functional characterization of a novel, arrestin-biased designer G protein-coupled receptor. Mol Pharmacol. 82:...

  2. Antibody Guide

    Type
    Guide
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...

  3. Molecular Biology Reference

    Type
    Guide
    ...Drives transcription of the insert. The promoter is designed to recruit transcriptional machinery from a particular...commonly used for plasmid selection Many plasmids are designed to include an antibiotic resistance gene, which...reaction. All the sequenced templates are then aligned to each other to assemble the entire sequence or...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the...new window) Kwon, I., & Schaffer, D. V. (2008). Designer gene delivery vectors: molecular engineering and...., Schaffer, D. V., & Karpova, A. Y. (2016). A designer AAV variant permits efficient retrograde access...

  5. Guide to Using Pooled Libraries

    Type
    Guide
    ...effects of expressing multiple genes at once. A well-designed screen can help you begin to understand what genes...tube. Pooled libraries can be small, if they are designed to cover only a subset of genes, or very large...

  6. CRISPR Guide

    Type
    Guide
    ...Increasing Cas9 PAM flexibility CRISPR systems designed for more precise edits require PAM sequences in...with base editing. While many base editors are designed to work in a very narrow window proximal to the...to target paralogs or lncRNAs . Guide RNAs are designed in silico and synthesized (Figure 8A), then cloned...

  7. Cloning

    Type
    Guide
    ...stranded DNA fragments can be used, so if properly designed, any insert fragment (PCR product or synthesized... backbone independent of how it was originally designed to be cloned into. A drawback of this system is...

  8. Plan Your Experiment

    Type
    Guide
    ... System Once you have selected your target and designed your gRNA(s), the next step is to choose how to...

  9. Adenovirus Guide

    Type
    Guide
    ...be harvested about 7–10 days later. Vogelstein designed multiple shuttle plasmids for different purposes...
Showing: 1 - 9 of 9 results