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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 18 results for: gne

Showing: 1 - 18 of 18 results

  1. AAV Production in HEK293 Cells

    Type
    Protocol
    ... Hazardous waste container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents...Fisher, 12605010) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment... + 60 mL of 5 M Sodium Chloride + 4 mL of 1 M Magnesium Chloride Close the bottle and mix by inverting...

  2. Protocol - Bacterial Transformation

    Type
    Protocol
    ...Because of this, nearly all plasmids (even those designed for mammalian cell expression) carry both a bacterial...cause the cells to take up the DNA, an electro-magnetic field is applied to the cell/DNA mixture to induce...

  3. Lentivirus Production

    Type
    Protocol
    ... Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning 10...Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

  4. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant... deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...

  5. CRISPR Library Amplification

    Type
    Protocol
    ...library during amplification. This protocol is designed to be as general as possible but note that individual...than Nanodrop, although most protocols have been designed with Nanodrop as the quantification method. If...

  7. Water Bath Protocol

    Type
    Protocol
    ... of bacteria or fungi. There are disinfectants designed to be used in water baths with instructions on...

  9. Fluorescence Titering Assay

    Type
    Protocol
    ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

  11. Colony Formation Titering Assay

    Type
    Protocol
    ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

  12. Pipetting Protocol

    Type
    Protocol
    ...of the pipette. Select the pipette tip that is designed to fit your pipette. To load a tip onto a pipette...

  16. Western Blot

    Type
    Protocol
    ...Top Stack such that the electrical contacts are aligned with the corresponding electrical contacts on the...

  18. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...
Showing: 1 - 18 of 18 results