We narrowed to 15,806 results for: nans

1. pNLF1W

   Plasmid

   #136404

   Purpose

   Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaRIgG-2

   Plasmid

   #228996

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-2
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaGIgG-12

   Plasmid

   #228999

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaGIgG-12
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGH00.0126

   Plasmid

   #64257

   Purpose

   Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

   Depositor

   Siela Maximova, Mark Guiltinan

   Article

   Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

   Inserts

   EGFP
   neomycin phosphotransferase II
   

   Use

   Binary agrobacterium vector for plant transformat…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   E12-omega

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAB1679 CMV-HIVNES-GS-Cas13bt3

   Plasmid

   #176317

   Purpose

   CMV-HIVNES-GS-Cas13bt3 (human codon optimized Cas13bt3 expression)

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

   Insert

   human codon optimized Cas13bt3
   

   Use

   CRISPR

   Tags

   HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPEP-TEV cNup98 (1-498)

   Plasmid

   #38037

   Depositor

   Roderick Lim

   Article

   Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

   Insert

   Nucleoporin 98 (NUP98 Human)
   

   Use

   Tags

   His-tag, 3Cysteine

   Expression

   Bacterial

   Mutation

   amino acids 1-498

   Promoter

   T7

   Available since

   Sept. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKII-Ace-mNeon2

   Plasmid

   #195526

   Purpose

   Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   Ace-mNeon2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Ace-mNeon 81S; SY linker

   Promoter

   CaMKII

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3-Flag-Tpr

   Plasmid

   #60882

   Purpose

   Expresses Flag-Tagged nucleoporin Tpr in Mammalian Cells

   Depositor

   Vinay Nandicoori

   Article

   Vomastek et al Mol Cell Biol. 2008 Nov;28(22):6954-66.

   Insert

   Tpr (TPR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-NBL10

   Plasmid

   #69971

   Purpose

   Cre-conditionally expresses the anti-GFP Nanobody-Rpl10a fusion

   Depositor

   Jeffrey Friedman

   Article

   Ekstrand et al Cell. 2014 May 22;157(5):1230-42. doi: 1

   Insert

   NBL10
   

   Use

   AAV and Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. B2342_BTB-mCherry

    Plasmid

    #215095

    Purpose

    Expresses BCL6 BTB(1-129)-mCherry in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    BCL6 BTB (BCL6 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-EPG

    Plasmid

    #117978

    Purpose

    Express EPG in mamalian cells

    Depositor

    Assaf Gilad

    Article

    Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

    Insert

    Electromagnetic perceptive gene
    

    Use

    Tags

    HIS and v5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn_CapChR2_mScarlet

    Plasmid

    #188039

    Purpose

    Expresses CapChR2-mScarlet in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

    Plasmid

    #195536

    Purpose

    Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKII-VARNAM2-Kv2.1PR

    Plasmid

    #195530

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CaMKII

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. B2396_BTB-EGFP

    Plasmid

    #215099

    Purpose

    Expresses BCL6 BTB(1-129)-EGFP in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    BTB-EGFP (BCL6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. (SUMO)10-(SIM)5

    Plasmid

    #126866

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-WT (AF41)

    Plasmid

    #21367

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. L-GARS-2

    Plasmid

    #166531

    Purpose

    scFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739

    Depositor

    Susanne Gräslund

    Article

    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-MYRIP

    Plasmid

    #89586

    Purpose

    Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYRIP (Myrip Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. nLuc-ΔECD-PTHR1

    Plasmid

    #190011

    Purpose

    Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    nLuc-ΔECD-PTHR1 (PTH1R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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