We narrowed to 2,547 results for: organization Addgene

1. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

   Plasmid

   #183410

   Purpose

   piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   KRAB-dCas9-ecDHFR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Tight-Puro-KrasG12D2B

   Plasmid

   #139786

   Purpose

   Lentiviral tet-inducible expression of KrasG12D (mouse)

   Depositor

   Joan Massague

   Article

   Su et al Nature. 2020 Jan;577(7791):566-571. doi:

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   G12D

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-tetON-GATA6-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204189

   Purpose

   Doxycycline inducible lentiviral vector for human GATA6 overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   GATA6 (GATA6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. TOM20*-SBP-GFP

   Plasmid

   #120173

   Purpose

   Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
   

   Tags

   SBP and eGFP

   Expression

   Mammalian

   Mutation

   Truncated TOM20: 1-30 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LAMP-mCherry-CRY2

   Plasmid

   #102249

   Purpose

   Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   LAMP-mCherry-CRY2 (LAMP1, CRY2 Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHDR_sox2HA_P2A-GFP-pA_HA_G2913A

   Plasmid

   #196191

   Purpose

   Tagging human SOX2 gene with P2A-EGFP at the C-terminus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   SOX2-HAL-P2A-EGFP-SOX2-HAR (SOX2 Human)
   

   Use

   Homology repair donor

   Mutation

   G2913A mutation to replace the guide cutting site…

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRPML1-mCherry

   Plasmid

   #135189

   Purpose

   Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

   Depositor

   Antony Galione

   Article

   Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

   Insert

   TRPML1 (Mcoln1 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. TVBB C-term-mScarlet-Blast

   Plasmid

   #169221

   Purpose

   Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Blast at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked mScarlet-P2A-Blast
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV 4xSTAR-mTagBFP2-blast

   Plasmid

   #125239

   Purpose

   Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

   Depositor

   Hugo Snippert

   Article

   Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

   Insert

   stem cell Ascl2 reporter, 4 repeats
   

   Use

   Lentiviral

   Tags

   mTagBFP2 (codon optimized)

   Expression

   Mammalian

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TVBB C-term-mScarlet-Puro

    Plasmid

    #169220

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mScarlet-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mScarlet-P2A-Puro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TVBB C-term-mNeongreen-Puro

    Plasmid

    #169228

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Puro
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-human HoxB4-IRES-YFP

    Plasmid

    #91889

    Purpose

    Retroviral expression of human HoxB4

    Depositor

    Brian Sorrentino, Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    HoxB4 (HOXB4 Human)
    

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Promoter

    MMLV gag

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCh-KIF17*-strep

    Plasmid

    #120167

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 17 (KIF17 Human)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF17: 1-509 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCh-KIF13A*-strep

    Plasmid

    #120166

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 13A (KIF13A Human)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF13A: 1-639 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-SBP-VAP-A*

    Plasmid

    #120175

    Purpose

    Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    VAMP associated protein A (VAPA Human)
    

    Tags

    EGFP and SBP

    Expression

    Mammalian

    Mutation

    Truncated VAP-A: 118-249

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCh-KIF5A*-strep

    Plasmid

    #120163

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5A (Kif5a Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5A: 1-559 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA5/avitag-apex2-v5-fkbp8

    Plasmid

    #154460

    Purpose

    expresses avitag-apex2-v5-fkbp8/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    avitag-apex2-v5-fkbp8 (FKBP8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV-F, T7 promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3/flag-pbira(1-g78)-sec61b

    Plasmid

    #154455

    Purpose

    expresses flag-pbira(1-g78)-sec61b/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    flag-pbira(1-g78)-sec61b (SEC61B Human)
    

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-HF1RA-P2A-GFP-PGK-Puro

    Plasmid

    #110866

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. TVBB C-term-mNeongreen-Blast

    Plasmid

    #169229

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Blast at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Blast
    

    Use

    Targeting vector backbone

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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