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DTECT - Plasmid for Standard Curve

Plasmid

#139333

Purpose

This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

DTECT ligation product

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pKeratin-miRFP703

Plasmid

#79990

Purpose

Keratin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Keratin (KRT18 Human)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2Q2

Plasmid

#124668

Purpose

Expresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2Q2 (UBE2Q2 Human)

Use

Tags

2xStrep/HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pDsRed2-C1-Ahi1

Plasmid

#30495

Depositor

Joseph Gleeson

Article

Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

Insert

Ahi1 (AHI1 Human)

Use

Tags

DsRed2

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pEF1α-squirrel-monkey-CHMP3(155)-HA

Plasmid

#154173

Purpose

expresses squirrel monkey CHMP3(155) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3(155)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 155

Promoter

EF1-alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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myc-hRAD18 C207F

Plasmid

#68829

Purpose

Expresses c-myc-tagged human RAD18 with C207F mutation

Depositor

Satoshi Tateishi

Article

Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

Insert

c-myc tagged human RAD18 with C207F mutation (RAD18 Human)

Use

Tags

c-myc

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Plasmid

#125229

Purpose

Expresses transmembrane intracellular NanoLuc in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pRShygro shID3#1

Plasmid

#19164

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP

Plasmid

#154185

Purpose

expresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Use

Tags

HA and T2A-H2B-BFP

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBV-Luc/Del-7

Plasmid

#14968

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-26/+334) (MYC Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-26/+334 (relative to the P2 transcription initia…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Tags

Expression

Mutation

Promoter

L. donovani ribosome RNA promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pRK-HA-TRAF4

Plasmid

#58314

Purpose

HA-tagged mouse TRAF4

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pIS1-wt RhoA 3'UTR

Plasmid

#26089

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

wt RhoA 3'UTR (RHOA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pBV-Luc/Frag-F

Plasmid

#14053

Depositor

Joan Massague

Article

Chen et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

Insert

c-myc promoter (-109/-3) (MYC Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-109/-3 (relative to the P2 transcription initiat…

Promoter

Available since

May 10, 2007

Availability

Academic Institutions and Nonprofits only
AcrIIC1X

Plasmid

#128112

Purpose

AcrX expression in mammalian cells; AcrX is an engineered anti-CRISPR protein targeting S. aureus Cas9

Depositor

Dominik Niopek

Article

Mathony et al Nat Chem Biol. 2020 Apr 13. pii: 10.1038

Insert

AcrX (AcrIIC1 N3F/D15Q/A48I)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TT S6K (E389-deltaCT)

Plasmid

#58507

Purpose

Gateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCT

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Rps6kb1 (Rps6kb1 Rat)

Use

Entry vector

Tags

HA

Expression

Mutation

T389E, stop codon at amino acid 402

Promoter

Available since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
R9-WT

Plasmid

#125210

Purpose

Expressing wildtype human Rad9b gene

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24-C840W shRescue

Plasmid

#28137

Depositor

Michelle Barton

Article

Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

C840Wmutated at aa 308 and 309 for shRNA rescue; …

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pXPR_sgGFP3

Plasmid

#119875

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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CS51

Plasmid

#117433

Purpose

Expression of ARL3

Depositor

Richard Kahn

Article

Cavenagh et al J Biol Chem. 1994 Jul 22;269(29):18937-4

Insert

ARL3 (ARL3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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