We narrowed to 140,984 results for: LAS

1. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

   Plasmid

   #192666

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-VP64, MS2-VP64
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1

   Plasmid

   #101177

   Purpose

   Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   hSpCas9-eSpCas9(1.1)-HF1(N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A)
   

   Use

   Tags

   3x FLAG and NLS (SV40)

   Expression

   Mammalian

   Mutation

   N497A/R661A/Q695A/K848A/Q926A/K1003A/R1060A

   Promoter

   CMV

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain

   Plasmid

   #101205

   Purpose

   Bacterial expression plasmid for SpCas9 REC3 domain

   Depositor

   Keith Joung, Jennifer Doudna

   Article

   Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

   Insert

   SpCas9 variant K506–Q712
   

   Use

   Tags

   10x His, MBP, and TEV site

   Expression

   Bacterial

   Mutation

   K506–Q712

   Promoter

   T7

   Available since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

   Plasmid

   #205542

   Purpose

   Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN1-MCP
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

   Plasmid

   #205547

   Purpose

   Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11-MCP-I
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

   Plasmid

   #205548

   Purpose

   Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11-MCP-p6
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOS030E: Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

   Plasmid

   #163071

   Purpose

   Entry vector for: ratiometric pHluorin (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   ratiometric pHluorin
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

   Plasmid

   #186745

   Purpose

   dCas9-10XGcn4-P2A-mCherry expression construct

   Depositor

   Richard Sherwood

   Article

   Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

   Insert

   Cas9-10XGcn4-P2A-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIE067 CAG mKate2 EF1a EBFP2-P2A-BlastR

   Plasmid

   #138747

   Purpose

   Integration Vector, assembled with pHIE041 (TUPV1), pJM451 (TUPV2), pPD612

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   CAG
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOS031E: Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

    Plasmid

    #163068

    Purpose

    Entry vector for: superecliptic pHluorin (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138739

    Purpose

    Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A

    Plasmid

    #182049

    Purpose

    expression of Sox2 mutants

    Depositor

    Hyonchol Jang

    Article

    Kim et al Exp Mol Med. 2021 Nov;53(11):1759-1768.

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S248A/T258A

    Promoter

    CAG

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant

    Plasmid

    #101202

    Purpose

    Bacterial expression plasmid for SpCas9∆REC3 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant M1–N497,GGS,V713–D1368
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    M1–N497, GGS, V713–D1368

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Plasmid

    #101233

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 2 variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant G582A/V583A/E584A/D585A/N588A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    G582A, V583A, E584A, D585A and N588A

    Promoter

    T7

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

    Plasmid

    #101213

    Purpose

    Bacterial expression plasmid for SpCas9 + K855A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant K855A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    K855A

    Promoter

    T7

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. MDH1-miR-196a-1SM-PGK-GFP-Blast

    Plasmid

    #31510

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a1 (Mir196a-1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMXS-IRES-BLAST flag-CAD H1734A H1741A

    Plasmid

    #169830

    Purpose

    Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    H1734A H1741A; TCCC -> AGTC silent mutations a…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXS-IRES-BLAST flag-CAD delta Loop1

    Plasmid

    #169831

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids F1366 - P1376; TCCC ->…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-BLAST flag-CAD delta Loop2

    Plasmid

    #169832

    Purpose

    Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids R1398 - S1407; TCCC ->…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXS-IRES-BLAST flag-CAD T456A S1406A

    Plasmid

    #169823

    Purpose

    Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

    Depositor

    Richard Possemato

    Article

    Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

    Insert

    CAD (CAD Human)
    

    Use

    Retroviral

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    T456A S1406A; TCCC -> AGTC silent mutations at…

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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