We narrowed to 6,239 results for: nls

1. pUC57-ITR2-H1- sgCTG Frodo-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210734

   Purpose

   Coding for SaSp D10A Cas9 alongside Frodo sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Frodo sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-ITR2-H1- sgCTG Gollum-eCMV-SaSpD10A (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210735

   Purpose

   Coding for SaSp D10A Cas9 alongside Gollum sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Gollum sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

   Plasmid

   #230075

   Purpose

   Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

   Depositor

   Sumeet Pal Singh

   Article

   Hovhannisyan et al bioRxiv 2025.05.23.655316

   Insert

   lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
   

   Use

   Cre/Lox; Zebrafish expression

   Promoter

   fabp10a

   Available Since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

   Plasmid

   #223287

   Purpose

   A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
   

   Use

   Synthetic Biology; Piggybac transposase insertabl…

   Expression

   Mammalian

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c

   Plasmid

   #210508

   Purpose

   expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
   

   Tags

   GB1

   Expression

   Mammalian

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

   Plasmid

   #210736

   Purpose

   Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Inserts

   SaSp D10A Cas9
   Merry sgCAG
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, PolyA signal

   Expression

   Mammalian

   Mutation

   D10A, N-terminal Sa Cas9, C-terminal Sp Cas9

   Promoter

   H1 and eCMV

   Available Since

   Jan. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a Gx-mNG-NL

   Plasmid

   #169106

   Purpose

   Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

   Depositor

   Maarten Merkx

   Article

   Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

   Insert

   protein G - mNeonGreen - NanoLuc
   

   Tags

   his-tag and strep-tag

   Expression

   Bacterial

   Mutation

   Ala 24 stop

   Promoter

   T7

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCD/NL-BH*DDD

   Plasmid

   #17531

   Depositor

   Jakob Reiser

   Article

   Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

   Insert

   HIV-1 Gag/Pol, Tat, Rev
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCD/NL-BH∆1

   Plasmid

   #41791

   Purpose

   Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev

   Depositor

   Jakob Reiser

   Article

   Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

   Insert

   HIV-1 Gag/Pol and RRE sequences
   

   Expression

   Mammalian

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 myc-NL-GW

    Plasmid

    #113446

    Purpose

    NanoLuc Gateway shuttle vector for N-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Luciferase; Gateway shuttle vector

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-NL-DHFR-SpCas9

    Plasmid

    #124522

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NL-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 GW-NL-myc

    Plasmid

    #113447

    Purpose

    NanoLuc Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Luciferase; Gateway shuttle vector

    Tags

    NanoLuc-cmyc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti myc-NL-GW

    Plasmid

    #113454

    Purpose

    Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    cmyc-NanoLuc

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330 NL-FKBP-Cas9

    Plasmid

    #162723

    Purpose

    Mammalian expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti GW-NL-myc

    Plasmid

    #113455

    Purpose

    Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Type

    Empty backbone

    Use

    Lentiviral and Luciferase

    Tags

    NanoLuc-cmyc

    Expression

    Mammalian

    Promoter

    hUbC

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti PA-mCit-NL

    Plasmid

    #113452

    Purpose

    Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    hUBbC

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 PA-mCit-NL

    Plasmid

    #113444

    Purpose

    ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

    Depositor

    Erich Wanker

    Article

    Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

    Insert

    ProteinA-mCitrine-NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCD/NL-BH*deltavpu/RT-

    Plasmid

    #136985

    Purpose

    Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

    Depositor

    Jakob Reiser

    Article

    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

    Insert

    HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
    

    Expression

    Mammalian

    Mutation

    carries D110E mutation in the reverse transcripta…

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCI Flag Smad3 NL EPSM

    Plasmid

    #27006

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-226; T179V S204A S208A S213A

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pET SUMO NL-FKBP-Cas9

    Plasmid

    #162730

    Purpose

    Bacterial expression, protein expression

    Depositor

    Amit Choudhary

    Article

    Sreekanth et al ACS Cent Sci. 2020 Dec 23;6(12):2228-223

    Insert

    FKBP F36V
    

    Tags

    6xHis and SUMO

    Expression

    Bacterial

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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