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pSKB3-SaGGR-F219L (JBEI-7186)

Plasmid

#110151

Purpose

pSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant gene

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Tags

TEV-cleavable His-tag

Expression

Bacterial

Mutation

SaGGR-F219L mutant gene

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PDAE

Plasmid

#110180

Purpose

Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase Activating Enzyme

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD

Plasmid

#110168

Purpose

Expresses propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pJK287

Plasmid

#71120

Purpose

Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

Depositor

T. J. Kappock

Article

Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

Insert

formyl-CoA:oxalate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pJK360

Plasmid

#71571

Purpose

Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

acetyl-CoA:oxalate CoA-transferase

Expression

Bacterial

Mutation

changes threonine-2 to alanine

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pJK385

Plasmid

#71572

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

succinyl-CoA:acetate CoA-transferase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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KHLH31 pEGFP-N2 (1089)

Plasmid

#62032

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KHLH31

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pFAB806

Plasmid

#47849

Purpose

BIOFAB reporter plasmid for measuring lambda tR2 termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

lambda tR2 terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO4

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB805

Plasmid

#47850

Purpose

BIOFAB reporter plasmid for measuring tonB termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tonB terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO5

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB816

Plasmid

#47851

Purpose

BIOFAB reporter plasmid for measuring ilvGEDA termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

ilvGEDA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO6

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB807

Plasmid

#47852

Purpose

BIOFAB reporter plasmid for measuring RNAI termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

RNAI terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO7

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB820

Plasmid

#47855

Purpose

BIOFAB reporter plasmid for measuring tetAC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

tetAC terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO10

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB1910

Plasmid

#47859

Purpose

BIOFAB reporter plasmid for measuring rpoC termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

rpoC terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO14

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB804

Plasmid

#47848

Purpose

BIOFAB reporter plasmid for measuring T3 early termination efficiency

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

T3 early terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLTetO3

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFAB3905

Plasmid

#47808

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Article

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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NSUN6

Plasmid

#188060

Purpose

Bacterial expression plasmid for human NSUN6; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN6 (NSUN6 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT_Dn29-dCas9-P2A-GFP

Plasmid

#247165

Purpose

In vitro transcription of human codon optimized fusion of Dn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Promoter

mutated T7

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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