We narrowed to 17,142 results for: tes

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pX462-hPRKAA2-gRNA_B

Plasmid

#74377

Purpose

gRNA_B to knockout human AMPK alpha 2 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Promoter

U6

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt-AH

Plasmid

#39533

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (PH domain only) (Akt1 Mouse)

Tags

6G linker and EGFP

Expression

Mammalian

Mutation

PH domain only

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
3XFlag pCMV5-TOPO TAZ ∂CC

Plasmid

#24816

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Mutation

Deletion of amino acids 222-265 (coiled coil doma…

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits
GB-Dcp1b

Plasmid

#153979

Purpose

Dimerization dependent fluorescent protein GB core domain fused to mRNA decapping enzyme 1b

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1b (DCP1B Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Tp-ecfp

Plasmid

#65034

Purpose

TpR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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GST-TAZ

Plasmid

#19024

Depositor

Michael Yaffe

Article

Park et al J Biol Chem. 2004 Apr 23. 279(17):17384-

Insert

TAZ (Wwtr1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
GFP-Paxillin WT

Plasmid

#186152

Purpose

For expressing paxillin variants in mammalian cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PXN (PXN Human)

Tags

mEGFP

Expression

Mammalian

Promoter

cmv

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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amilGFP chromoprotein

Plasmid

#117842

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-Incenp wt

Plasmid

#108487

Purpose

expression of vsv-INCENP wt-mCherry

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

INCENP (INCENP Human)

Tags

VSV and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-shRNA_Tet3

Plasmid

#85740

Purpose

shRNA against mouse Tet3

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

Tet3 (Tet3 Mouse)

Use

AAV

Tags

EYFP

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70

Plasmid

#62898

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-BHLHE40-IRES-ZsGreen1

Plasmid

#153061

Purpose

Lentiviral vector expressing human BHLHE40

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

BHLHE40 (BHLHE40 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-hNF2

Plasmid

#107150

Purpose

mammalian expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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GST-mDia1(FH1FH2DAD)-His

Plasmid

#85822

Purpose

contains the formin homolgy domains: FH2 is required for nucleation, processive tracking of barbed-end of actin filaments ; FH1 permits enhancement of actin polymerization rate.

Depositor

Antoine Jégou, Guillaume Romet-Lemonne

Article

Jegou et al Nat Commun. 2013;4:1883. doi: 10.1038/nc

Insert

Protein diaphanous homolog 1 (Diaph1 Mouse)

Tags

8*His and GST

Expression

Bacterial

Promoter

tac

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Rab21-T31N (DN)

Plasmid

#83423

Purpose

Mouse Rab21 mutant that has defects in the binding of GTP (dominant negative). Generated from pEGFP-Rab21 by site-directed mutagenesis.

Depositor

Johanna Ivaska

Article

Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.

Insert

Rab21 (Rab21 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Residue 31 from T to N (T31N: ACG -> AAC)

Promoter

CMV

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
MDH1-PGK-GFP-miR9

Plasmid

#25036

Depositor

Bob Weinberg

Article

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

mir-9-3 (MIR9-3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGs-NLS-TIR1_P2A_NES-TIR1

Plasmid

#117699

Purpose

Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

TRANSPORT INHIBITOR RESPONSE 1
TRANSPORT INHIBITOR RESPONSE 1

Tags

HA tag, Myc-NES, and NLS

Expression

Mammalian

Mutation

codon optimized for murine expression

Promoter

None, 2A peptide from porcine teschovirus-1 polyp…

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pVQ CMV NanoV1Mutant-2a-EGFP ferritin

Plasmid

#79650

Purpose

Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

Depositor

Jeffrey Friedman

Article

Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

Inserts

transient receptor potential vanilloid 1 (Trpv1 Rat)
camelid anti-GFP nanobody
Enhanced green fluorescent protein
mouse ferritin light chain (Ftl1 Mouse)
mouse ferritin heavy chain (Fth1 Mouse)

Use

Adenoviral

Expression

Mammalian

Mutation

isoleucine 679 changed to lysine

Promoter

CMV

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCSC-NLS-RFP

Plasmid

#221813

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear localization sequence (NLS)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NLS-mCherry

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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