We narrowed to 3,710 results for: ubiquitin

1. CAV1-K*R-HA

   Plasmid

   #27765

   Depositor

   Ari Helenius

   Article

   Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

   Insert

   caveolin-1, lysine null mutant (CAV1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   All lysines mutated to arginines (K*R)

   Promoter

   Available since

   Aug. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO-FNTA-FLAG

   Plasmid

   #86858

   Purpose

   To express FNTA with a FLAG tag at C terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   FNTA (FNTA Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   The sequence of the insert is isoform 2, which di…

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA SF-HERC2 F1

   Plasmid

   #55784

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pETNKI-his-SUMO-RNF168ring (1-189)

   Plasmid

   #63570

   Purpose

   Expression of human RNF168 ring domain (residues 1-189) tagged with His-SUMO

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   6xHis and SUMO3

   Expression

   Bacterial

   Mutation

   Ring domain only (residues 1-189)

   Promoter

   T7

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pGEX-parkin 141-465

   Plasmid

   #45971

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin 141-465 (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Dele…

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pQCXIH-Flag Smad3 WT

   Plasmid

   #27025

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   Smad3 (SMAD3 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pCI-Neo-gp78/JM20

   Plasmid

   #13303

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA SF-HERC2 F3

   Plasmid

   #55786

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1700-2700aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (1700aa-2700aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pRK5-Flag-HA-Merlin

   Plasmid

   #27104

   Depositor

   Filippo Giancotti

   Article

   Li et al Cell. 2010 Feb 19. 140(4):477-90.

   Insert

   Merlin (Nf2 Mouse)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCS2 Flag Smad3 EPSM V179T

    Plasmid

    #26998

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Xenopus/mammalian/avian/zebrafish

    Tags

    Flag

    Expression

    Mutation

    T179V S204A S208A S213A; V179T

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCDNA3.1-Flag-H2A K13R

    Plasmid

    #63566

    Purpose

    Expression of human H2A with K13R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 13 is now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3.1-Flag-H2A K15R

    Plasmid

    #63567

    Purpose

    Expression of human H2A with K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine at 15 is now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

13. FUG-T2A-Cas9

    Plasmid

    #75346

    Purpose

    Ubiquitin promoter expresses GFP and humanized spCas9 (from PX330) via a T2A motif. For cell transfection or use in lentiviral packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

    Depositor

    Bryan Luikart

    Article

    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP (via t2a)

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCI Flag Smad3 LC EPSM

    Plasmid

    #27038

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 133-425; T179V S204A S208A S213A

    Promoter

    Available since

    Jan. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pRK5-Merlin-Flag-HA

    Plasmid

    #27103

    Depositor

    Filippo Giancotti

    Article

    Li et al Cell. 2010 Feb 19. 140(4):477-90.

    Insert

    Merlin (Nf2 Mouse)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3flag-RanGAPdeltaN

    Plasmid

    #25104

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    RanGAP1 deleted N-term domain (RANGAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-203.

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-WRAP53beta

    Plasmid

    #64676

    Purpose

    expresses Wild type WRAP53beta

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    WD40-encoding RNA antisense to P53 (WRAP53 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCI Flag Smad3 NL EPSM

    Plasmid

    #27006

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    Smad3 (SMAD3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    aa 1-226; T179V S204A S208A S213A

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

19. p4979 pcDNA4-E6AP III L502P

    Plasmid

    #32892

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Use

    Tags

    6xHis Express and T7-tag

    Expression

    Mammalian

    Mutation

    L502P

    Promoter

    CMV

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only

20. p4978 pcDNA4-E6AP III E550L

    Plasmid

    #32891

    Depositor

    Peter Howley

    Article

    Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

    Insert

    E6AP (UBE3A Human)
    

    Use

    Tags

    6xHis Express and T7-tag

    Expression

    Mammalian

    Mutation

    E550L

    Promoter

    CMV

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only