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pCAGGS-4591NES

Plasmid

#138377

Purpose

Booster-ROCK, a Red-Shifted Genetically Encoded FRET Biosensor for ROCK activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-ROCK

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-Halo7Tag

Plasmid

#111135

Purpose

Tom20 with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

Receptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)

Tags

Halo7 Tag

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

Plasmid

#86230

Purpose

Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

Depositor

Benoit Bruneau

Article

Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

Insert

AID[71-114]-eGFP

Use

Mouse Targeting

Expression

Mammalian

Available Since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO hChR2(E123A)-EYFP

Plasmid

#35507

Purpose

AAV expression of EF1a-driven, cre-dependent, hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

hChR2

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123A

Promoter

Ef1a

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

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pSEMS-Halo7Tag-hFis

Plasmid

#111136

Purpose

hFis with selflabeling tag, e.g. for fluorescence microscopy

Depositor

Karin Busch

Article

Appelhans et al Nano Lett. 2012 Feb 8;12(2):610-6. doi:

Insert

human fission factor1 (FIS1 Human)

Tags

Halo7-tag

Expression

Mammalian

Mutation

*

Promoter

CMV

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-CMV-intron-CasRx-pA

Plasmid

#192485

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMT-Mef2c-tPT2A-GFP

Plasmid

#111771

Purpose

Bicistronic construct: Co-express two genes by 2A peptides

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Insert

Mef2c-tPT2A-GFP (Mef2c Mouse)

Use

Cloning intermediate

Tags

GFP

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-mPGK-eGFP (beta-globin intron)

Plasmid

#162513

Purpose

AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

Depositor

Bart Nieuwenhuis

Article

Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

Insert

eGFP

Use

AAV

Expression

Mammalian

Promoter

mPGK

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-COV M

Plasmid

#165124

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

COV M (M codon optimized SARS-COV-2)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 barr1 flag

Plasmid

#14687

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

beta arrestin 1 (Arrb1 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only