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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 25,285 results for: crispr

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Showing: 2161 - 2180 of 25285 results

  2. AA183

    Plasmid
    #216025
    Purpose
    Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9 (H840A)_v2.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216025-plasmid-map-sequence-id-431472

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  3. AA028

    Plasmid
    #216008
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for base editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9 (D10A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216008-plasmid-map-sequence-id-431426

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  4. AA060

    Plasmid
    #216017
    Purpose
    Fragmid fragment: (Cas protein) nickase Cas for prime editing
    Depositor
    John Doench
    Publication
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nCas9 (H840A)_v1.1 [Sp]
    Use
    CRISPR; Fragment
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216017-plasmid-map-sequence-id-431396

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  8. pKS7107

    Plasmid
    #89051
    Purpose
    Expresses Nm crRNA, Nm tracrRNA and human codon-optimized NmCas9
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Nm crRNA
    Nm tracrRNA
    hNmCas9
    Use
    CRISPR
    Tags
    HA tag, NLS, and SV40 NLS
    Promoter
    EF1a and human U6
    Available since
    April 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    89051-plasmid-map-sequence-id-176334

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  9. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    193967-plasmid-map-sequence-id-381640

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  10. pSLQ10844

    Plasmid
    #183956
    Purpose
    CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, Hygromycin Resistance, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    183956-plasmid-map-sequence-id-363081

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  11. pcDNA3-Flag::UbvG08 I44A, deltaGG

    Plasmid
    #74939
    Purpose
    Ubiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1
    Depositor
    Daniel Durocher
    Publication
    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ubiquitin
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UbvG08 with I44A mutation and no terminal Glycines
    Promoter
    CMV
    Available since
    June 30, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    7 Articles
    74939-plasmid-map-sequence-id-151347

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  13. AICSDP-23:TJP1-mEGFP

    Plasmid
    #87429
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TJP1 Homology Arms with mEGFP-linker (TJP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    87429-plasmid-map-sequence-id-174170

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  14. pCMV-PE6e

    Plasmid
    #207855
    Purpose
    Mammalian expression of PE6e prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6e
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Cas9(H840A, K918A, K775R)
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    207855-plasmid-map-sequence-id-408162

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  16. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

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  17. pMuLE ENTR CMV-hCas9 R4-R3

    Plasmid
    #62132
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing CMV promoter and human codon optimized Cas9 module Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    62132-plasmid-map-sequence-id-101416

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  18. pMuLE ENTR SV40-hCas9 L5-L2

    Plasmid
    #62134
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Insert
    human codon optimized Cas9
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Feb. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    62134-plasmid-map-sequence-id-101418

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  20. PX400 Campylobacter lari CF89-12 Cas9

    Plasmid
    #68336
    Purpose
    Campylobacter lari CF89-12 Cas9
    Depositor
    Feng Zhang
    Insert
    ClCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    68336-plasmid-map-sequence-id-119558

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Showing: 2161 - 2180 of 25285 results