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ptac::ccdB

Plasmid

#15922

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

ccdB (ccdB E. coli)

Expression

Bacterial

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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pRetroSuper Smad4

Plasmid

#15727

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad4 shRNA (SMAD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pAd-Track Flag GCN5 Y621A/P622A

Plasmid

#14425

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 Y621A/P622A (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

Y621A, P622A. Catalytically inactive acetyltransf…

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pSICO-Flpo

Plasmid

#24969

Depositor

Tyler Jacks

Article

Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

Insert

Flp recombinase

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#29582

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk2 (Mapk1 Rat)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met removed from Erk2

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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p3998 pGEX HHR23A

Plasmid

#10864

Depositor

Peter Howley

Article

Kumar et al J Biol Chem. 1999 Jun 25. 274(26):18785-

Insert

HHR23A (RAD23A Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
TRX1 His C32S C35S

Plasmid

#21286

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Tags

His

Expression

Mammalian

Mutation

C32S C35S

Available Since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2E2

Plasmid

#15788

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E2 (UBE2E2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2G1

Plasmid

#15790

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pCR4-huNFATc1nuc

Plasmid

#23988

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1 (NFATC1 Human)

Use

Cloning vector

Tags

EE tag

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-TrxRFP1

Plasmid

#98996

Purpose

mammalian expression of a biosensor to monitor thioredoxin redox dynamics

Depositor

Huiwang Ai

Article

Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

Insert

human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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hRIP3 GFP D160N

Plasmid

#41386

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Kinase Domain Mutant (D160N)

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-FDD

Plasmid

#80629

Purpose

Fluorescent destablizing domain for regulating protein stability and fluorescence

Depositor

Thomas Wandless

Article

Navarro et al ACS Chem Biol. 2016 Jun 6.

Insert

UnaG

Use

Retroviral

Tags

HA-mcherry-linker

Mutation

mutations A36V, R136G

Promoter

LTR

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-Y1699C

Plasmid

#25364

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pDEST51-LRRK2-R1441C

Plasmid

#25081

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-PKAKTRClover

Plasmid

#59146

Purpose

ORF encoding PKA KTR mClover flanked by gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA Kinase Translocation Reporter (PRKACA Mouse, Human)

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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DYSF-3HA

Plasmid

#29767

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF (DYSF Human)

Tags

3xHA

Expression

Bacterial and Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA6-N-Myc-SCAP

Plasmid

#134285

Purpose

Expresses Myc-tagged SCAP in mammalian cells

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

SCAP (Scap, Scap Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBS mNFATc3-EE

Plasmid

#17868

Depositor

Jerry Crabtree

Article

Graef et al Cell. 2001 Jun 29. 105(7):863-75.

Insert

NFATc3 (Nfatc3 Mouse)

Tags

EE

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only