Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 3,177 results for: FRI;

Showing: 401 - 440 of 3177 results

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pET21-pelB-LaG94-9

Plasmid

#228968

Purpose

For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-10

Plasmid

#228969

Purpose

For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-12

Plasmid

#228970

Purpose

For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-3

Plasmid

#228963

Purpose

For bacterial expression of anti-GFP nanobody LaG94-3, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-3

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-8

Plasmid

#228967

Purpose

For bacterial expression of anti-GFP nanobody LaG94-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-6

Plasmid

#228966

Purpose

For bacterial expression of anti-GFP nanobody LaG94-6, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-6

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-18

Plasmid

#228972

Purpose

For bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-18

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-2

Plasmid

#228975

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaGIgG-4

Plasmid

#228998

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-2

Plasmid

#228962

Purpose

For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-1

Plasmid

#228961

Purpose

For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-9

Plasmid

#228993

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-8

Plasmid

#228992

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-8, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-10

Plasmid

#228978

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-10

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-4

Plasmid

#228964

Purpose

For bacterial expression of anti-GFP nanobody LaG94-4, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-8

Plasmid

#228976

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-8

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-9

Plasmid

#228977

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-9

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mCh-Drp1

Plasmid

#49152

Purpose

mammalian expression of Drp1 fused to mCherry

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

Drp1 (DNM1L Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Venus-Akaluc (neo)

Plasmid

#124701

Purpose

Aka-Luciferase reporter

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Akaluciferase

Use

Lentiviral

Promoter

hPGK

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-HRE-dUnaG

Plasmid

#124372

Purpose

UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

Depositor

Roland Friedel

Article

Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

Insert

HRE-dUnaG

Use

Lentiviral

Tags

PEST-degron and Myc tag

Promoter

HRE (HIF responsive element 5x + CMV minimal prom…

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI-N1ICD

Plasmid

#185525

Purpose

Constitutive overexpression of human NOTCH1 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH1 intracellular domain (NOTCH1 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GSK3betaS9A (1016)

Plasmid

#49492

Purpose

expresses human Gsk3beta with S9A (constitutively active) mutation

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

GSK3beta S9A (GSK3B Human)

Expression

Mammalian

Mutation

S9A (constitutively active)

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ADAR2DD_E488Q

Plasmid

#205598

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

ADAR2 deaminase domain (ADARB1 Human)

Expression

Mammalian

Mutation

E488Q hyperactive mutation

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_T6B -mCherry

Plasmid

#205600

Purpose

expresses human T6B peptide fused with mChery in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ADAR2DD_E488Q_Ago2

Plasmid

#205599

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

Depositor

Marc Friedlander

Article

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

hAgo2 (AGO2 Human)

Tags

ADAR2DD_E488Q (hyperactive mutant)

Expression

Mammalian

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FUG-Pten

Plasmid

#140732

Purpose

Lentiviral vector to overexpress wild-type human PTEN as a GFP-PTEN fusion protein

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-Pten (PTEN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

hUbiC

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWPI-N3ICD

Plasmid

#185524

Purpose

Constitutive overexpression of human NOTCH3 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH3 intracellular domain (NOTCH3 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FERM domain of Ezrin

Plasmid

#62380

Purpose

Expresses the FERM domain of Ezrin (N terminal portion of the protein)

Depositor

Guillaume Charras

Article

Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

Insert

Ezrin aa1-309 (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

contains FERM domain of Ezrin (N terminal portion…

Promoter

CMV

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Ad-EphA3 KD

Plasmid

#102726

Purpose

Expression of Kinase dead EphA3

Depositor

Jin Chen

Article

Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

Insert

EphA3 (Epha3 Mouse)

Use

Adenoviral

Mutation

Kinase Dead (K657M; AAA > ATG)

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV mSin1.1 HA

Plasmid

#12582

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 22, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.2 myc

Plasmid

#12577

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.5 myc

Plasmid

#12579

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.5 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.4 myc

Plasmid

#12578

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.4 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pMSCV mSin1.1 myc

Plasmid

#12576

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.1 (MAPKAP1 Human)

Use

Retroviral

Tags

myc

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
FUG-T2A-Pten

Plasmid

#140738

Purpose

Lentiviral vector to overexpress human PTEN and GFP

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTA-PTEN (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV mSin1.2 HA

Plasmid

#12583

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Insert

mSin1.2 (MAPKAP1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Nov. 30, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-IV-GFPL10

Plasmid

#98704

Purpose

Cre-dependently express GFPL10 fusion using Introvert Switch

Depositor

Jeffrey Friedman, Alexander Nectow

Article

Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

Insert

GFPL10

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Mutation

L10: 2609 C->T to remove EcoRI site

Promoter

EF1a

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLB2 5'3'

Plasmid

#49346

Purpose

luciferase reporter with Bmp2 promoter (1702 bp) and Bmp2 3'UTR (2015 bp)

Depositor

Melissa Rogers

Article

Fritz et al J Biol Chem. 2004 Nov 19;279(47):48950-8

Inserts

Bmp2 distal promoter (Bmp2 Mouse)
Bmp2 3' UTR (Bmp2 Mouse)

Use

Luciferase

Tags

luciferase

Promoter

-

Available Since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Plxnb2-ISH-2kb

Plasmid

#68036

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAS-CFP-MEK

Plasmid

#42077

Depositor

Norbert Perrimon

Article

Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

Insert

Drosophila MEK ortholog (Dsor1 Fly)

Tags

CFP

Expression

Insect

Promoter

Hsp70

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Showing: 401 - 440 of 3177 results

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