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Plasmid#209322PurposeAAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTHmut-EGFP
Plasmid#209323PurposeAAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.DepositorInsertAPOBEC1--YTHmut-HA
UseAAVTagsHAExpressionMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTH
Plasmid#209319PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTHmut
Plasmid#209320PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.DepositorInsertAPOBEC1-YTHmut-HA
UseAAVTagsHAExpressionMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA ins:Apollo-NADP+ mVenus
Plasmid#210518PurposeExpresses mVenus-tagged Apollo-NADP+ in zebrafish pancreatic beta cells using a zebrafish insulin promoterDepositorInsertApollo-NADP+
UseZebrafish expression, tol2 kit gateway-compatibleTagsmVenusExpressionMutationPromoterZebrafish insulin promoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-APOBEC1-YTH-mCherry
Plasmid#197035PurposeExpress APOBEC1 cytosine deaminase fused with YTH m6A binding domain from UbiC promoter.DepositorInsertAPOBEC1-YTH
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_D125I_47-300_mCh-SspB (pBS1071)
Plasmid#185294PurposeFor the mammalian expression of the human protein ApoE3_D125I_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE3_D125I_47-300
UseTagsExpressionMammalianMutationD125IPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)
Plasmid#185309PurposeFor the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_D125I_47-300
UseTagsExpressionMammalianMutationD125IPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_47-300_mCh-2xFKBP (pBS1113)
Plasmid#185308PurposeFor the mammalian expression of the human protein ApoE3_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE4_47-300_mCh-2xFKBP (pBS1112)
Plasmid#185307PurposeFor the mammalian expression of the human protein ApoE4_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE4_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE2_47-300_mCh-2xFKBP (pBS1111)
Plasmid#185306PurposeFor the mammalian expression of the human protein ApoE2_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE2_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_47-300_mCh-SspB (pBS1070)
Plasmid#185293PurposeFor the mammalian expression of the human protein ApoE3_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE3_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE4_47-300_mCh-SspB (pBS1069)
Plasmid#185292PurposeFor the mammalian expression of the human protein ApoE4_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE4_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE2_47-300_mCh-SspB (pBS1068)
Plasmid#185291PurposeFor the mammalian expression of the human protein ApoE2_47-300 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE2_47-300
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP humanAPOBEC1 L173A/G227A
Plasmid#167171PurposeExpresses human APOBEC1 L173A/G227A in mammalian cellsDepositorArticleInserthumanAPOBEC1 (APOBEC1 Human)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TAL-apobb.1-PR1-RA
Plasmid#128694PurposeRight arm of TALEN pair 1 for cleavage of apobb.1 STOP locusDepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-TAL-apobb.1-PR1-LA
Plasmid#128693PurposeLeft arm of TALEN pair 1 for cleavage of apobb.1 STOP locusDepositorInsertTALEN
UseTALENTagsExpressionMutationPromoterAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX
Plasmid#163543PurposeMammalian CG-to-GC base editingDepositorInsertPOLD2-APOBEC1-UdgX-nCas9-UdgX
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pST1374-scFv-APOBEC-UGI-GB1
Plasmid#113029PurposeExpresses scFv-rAPOBEC1-UGI in mammalian cellsDepositorInsertscFv-rAPOBEC1-UGI-GB1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-APOBEC-nCas9-Ung
Plasmid#198552PurposeExpresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-APOBEC-nCas9-Ung
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only