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pMWCAVI_H6BAP-OROV_N

Plasmid

#240162

Purpose

Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…

Expression

Bacterial

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOPTH OROV N

Plasmid

#229663

Purpose

Express Oropouche virus BeAn 19991 nucleoprotein with N-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

Plasmid

#74919

Purpose

Mammalian expression of mKlf2 and mNanog-mCherry

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Inserts

Klf2 (Klf2 Mouse)
Nanog (Nanog Mouse)

Tags

T2A and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only
pTN182

Plasmid

#34888

Depositor

Mitsuyasu Hasebe

Article

Sakakibara et al Evol Dev. 2008 Sep-Oct;10(5):555-66.

Type

Empty backbone

Use

Moss targeting

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
PB CRIV Gc Spike H6

Plasmid

#240163

Purpose

Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

CRIV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 478-906 only

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED4(130-184) + CBP TAZ1(340-439)

Plasmid

#254288

Purpose

Bacterial coexpression of human CITED4 CTAD with CBP TAZ1

Depositor

Rebecca Berlow

Article

Do et al bioRxiv [Preprint]. 2026 Jan 21:2026.01.

Inserts

CITED4 (130-184) (CITED4 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Tags

6X His-tagged GB1

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED1(146-193) + CBP TAZ1(340-439)

Plasmid

#254286

Purpose

Bacterial coexpression of human CITED1 CTAD with CBP TAZ1

Depositor

Rebecca Berlow

Article

Do et al bioRxiv [Preprint]. 2026 Jan 21:2026.01.

Inserts

CITED1 (146-193) (CITED1 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Tags

6X His-tagged GB1

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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Mouse Liver CRISPR Knockout Library

Pooled Library

#192824

Purpose

Knockout library targeting 13,189 of the 13,266 protein-coding genes expressed in the mouse liver.

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pQC

Plasmid

#221134

Purpose

Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator

Depositor

Daniel Daley

Article

Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102

Inserts

[PibpA-gfpASV]
[PcpxPmcherryASV] OPT

Expression

Bacterial

Promoter

cpxP and ibpA

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGSH0

Plasmid

#45500

Depositor

Nelson Lau

Article

Zeng et al Methods. 2013 May 23. pii: S1046-2023(13

Type

Empty backbone

Use

RNAi

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2013

Availability

Academic Institutions and Nonprofits only

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PB OROV Gc Spike H6

Plasmid

#229662

Purpose

Make PiggyBac stable cell line expressing Oropouche virus BeAn 19991 Gc spike region (residues 482–894 of M polyprotein) with C-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

OROV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 482-894 only

Promoter

TRE

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pALOD4

Plasmid

#111026

Purpose

Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

Depositor

Arun Radhakrishnan

Article

Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

Insert

Domain 4 of Anthrolysin O

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: S404C, C472A

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pTarKO

Plasmid

#110540

Purpose

one-step modification of chromosomal locous using targocil/tarO gene in Staphylococcus aureus

Depositor

Suzanne Walker

Article

Lee et al ACS Infect Dis. 2018 Mar 23. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology; Targeting vector for locus of …

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-mEos3.2

Plasmid

#189720

Purpose

titratable expression of mEos3.2 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mEos3.2

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-sfGFP

Plasmid

#189675

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-mNG

Plasmid

#189722

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIRSTD)-sfGFP

Plasmid

#189674

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIRSTD)-mNG

Plasmid

#189721

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-PAmCherry1

Plasmid

#189718

Purpose

titratable expression of PAmCherry1 in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

PAmCherry1

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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