Skip to main content
Addgene
Showing: 201 - 220 of 9790 results

  1. pRDAX-EMMI-036 Vimentin(D-G)//Esp3I

    Plasmid
    #130238
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    Vimentin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130238-plasmid-map-sequence-id-249616

    Enlarge

  2. pRDAX-EMMI-037 NLSNoLS(D-G)//Esp3I

    Plasmid
    #130239
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    NLSNoLS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130239-plasmid-map-sequence-id-249617

    Enlarge

  3. pRDAX-EMMI-038 Lyn(D-G)//Esp3I

    Plasmid
    #130240
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    Lyn
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130240-plasmid-map-sequence-id-249618

    Enlarge

  4. pRDAX-EMMI-039 LifeAct(D-G)//Esp3I

    Plasmid
    #130241
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    LifeAct
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130241-plasmid-map-sequence-id-249619

    Enlarge

  5. pRDAX-EMMI-017 IRES-WT(H-I)//Esp3I

    Plasmid
    #130219
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES wt
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130219-plasmid-map-sequence-id-249597

    Enlarge

  6. pRDAX-EMMI-034 EB3(D-G)//Esp3I

    Plasmid
    #130236
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    EB3
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130236-plasmid-map-sequence-id-271316

    Enlarge

  7. PIESP15-COMP-blac-flag-his

    Plasmid
    #110958
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    parasite-infected erythrocyte surface protein (PIESP15) (PF3D7_0103900 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    110958-plasmid-map-sequence-id-223542

    Enlarge

  9. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid
    #182653
    Purpose
    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    Use
    Tags
    HA tag and nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    E55D and Y306A/Y384F (AF) double mutant of Methan…
    Promoter
    CMV and U6
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    182653-plasmid-map-sequence-id-363026

    Enlarge

  11. BPK3258 - human expression plasmid for eSpCas9(1.1)

    Plasmid
    #101176
    Purpose
    Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
    Depositor
    Keith Joung, Jennifer Doudna
    Article
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)
    Use
    Tags
    3x FLAG and NLS (SV40)
    Expression
    Mammalian
    Mutation
    K848A/K1003A/R1060A
    Promoter
    CMV
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    101176-plasmid-map-sequence-id-193999

    Enlarge

  13. pRDAX-EMMI-021 IRES-D[C333G](H-I)//Esp3I

    Plasmid
    #130223
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-D[C333G]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C333G
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130223-plasmid-map-sequence-id-249601

    Enlarge

  14. pRDAX-EMMI-023 IRES-F[C336A](H-I)//Esp3I

    Plasmid
    #130225
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-F[C336A]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C336A
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130225-plasmid-map-sequence-id-249603

    Enlarge

  16. pRDAX-EMMI-018 IRES-A[G338A](H-I)//Esp3I

    Plasmid
    #130220
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-A[G338A]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    G338A
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130220-plasmid-map-sequence-id-249598

    Enlarge

  17. pRDAX-EMMI-019 IRES-B[C335T](H-I)//Esp3I

    Plasmid
    #130221
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-B[C335T]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    C335T
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130221-plasmid-map-sequence-id-249599

    Enlarge

  18. pRDAX-EMMI-020 IRES-C[A334G](H-I)//Esp3I

    Plasmid
    #130222
    Purpose
    EMMA Kit # 1000000119 extension
    Depositor
    Gary Lewin
    Insert
    IRES-C[A334G]
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    A334G
    Promoter
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    130222-plasmid-map-sequence-id-249600

    Enlarge

Showing: 201 - 220 of 9790 results