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Human messenger-RBP sgRNA library

Pooled Library

#168791

Purpose

Knockout library targeting human mRNA binding proteins with 10 sgRNAs/gene.

Depositor

Martin Turner

Article

Turner et al CRISPR J. 2021 Jun;4(3):427-437. doi: 10

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only
pCORE-UH

Plasmid

#72239

Purpose

Contains the CORE cassette with KlURA3 and hyg markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
hyg

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCORE-UK

Plasmid

#72238

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
kanMX4

Expression

Bacterial

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-melR-deGFP-T500

Plasmid

#45396

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

melibiose repressor

Expression

Bacterial

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pBADmod1-linker-deGFP

Plasmid

#45397

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Expression

Bacterial

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pB33eCPX

Plasmid

#23336

Depositor

Patrick Daugherty

Article

Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

Insert

Enhanced Circularly Permuted Outer Membrane Protein X

Expression

Bacterial

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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SW5

Plasmid

#40108

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Bistable switch using dynamic degradation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pChlr-pNEB193

Plasmid

#52136

Purpose

A backbone plasmid for creation of additional PUF repeat modules

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Type

Empty backbone

Expression

Bacterial

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ8001 - pCMV-R8.91 (D64V)

Plasmid

#202687

Purpose

R8.91 plasmid with mutated integrase (D64V)

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

gag pol

Use

Lentiviral

Expression

Mammalian

Mutation

D64V

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBEST-p15A-PLtetO1-tetR-T500

Plasmid

#45391

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

tet repressor

Expression

Bacterial

Promoter

PL-tetO1

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pSLQ8079 - IL2RG GFP-P2A CLIP Donor

Plasmid

#202690

Purpose

CLIP donor template for integrating GFP into IL2RG locus.

Depositor

Stanley Qi

Article

Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

Insert

CopGFP

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEM7063

Plasmid

#214001

Purpose

PrpsM driven expression of ssrA-tagged eGFP (LVA)

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

eGFP-LVA

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pZS2-123

Plasmid

#26598

Depositor

Michael Elowitz

Article

Cox RS et al J Biol Eng. 2010 Jul 21;4:10. doi: 10.11

Insert

Synthetic

Expression

Bacterial

Mutation

N/A

Available Since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

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pEM8731

Plasmid

#213991

Purpose

PrpsM driven expression of mNeonGreen - sequence codon-optimized for Salmonella

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mNeonGreen

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM10686

Plasmid

#213995

Purpose

PrpsM driven expression of mScarlet-I

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mScarlet-I

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pIYC04

Plasmid

#64741

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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PLlacO-1-mfLon

Plasmid

#45152

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lon

Use

Synthetic Biology

Tags

ssRA degradation (LVA)

Expression

Bacterial

Promoter

PLlacO-1

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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PLlacO-1-CImf

Plasmid

#40126

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lac inducible lambda repressor

Use

Synthetic Biology

Tags

mf-lon ssrA tag

Expression

Bacterial

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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pEM8317

Plasmid

#213989

Purpose

PrpsM driven expression of sfGFP

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

sfGFP

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEM8315

Plasmid

#213988

Purpose

PrpsM driven expression of mCherry2-L

Depositor

Marc Erhardt

Article

Delgadillo-Guevara et al J Biol Eng. 2024 Apr 8;18(1):25. doi: 10

Insert

mCherry2-L

Expression

Bacterial

Promoter

PrpsM

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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