We narrowed to 1,958 results for: HTA
-
Plasmid#64731PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNAR2
TagsCerulean3, N-terminal 30 amino acids from DAKAP1,…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-RAB-EKARev
Plasmid#118477PurposeddRFP-based single-color ERK biosensor targeted to the plasma membrane.DepositorInsertLyn-RAB-EKARev
TagsN-terminal targeting sequence from Lyn-kinaseExpressionMammalianPromoterCMVAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
nTRb412-gs10-NLS-VPR
Plasmid#188267PurposePlasmid ensures constitutive expression of the N-terminal fragment of split TRbeta (aa 200-412), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnTRb412 (TXNRD2 Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-NES
Plasmid#118407PurposeCytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-NES
TagsNuclear export signal (NES)ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEn-C1.1
Plasmid#61479PurposeEncodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPCDepositorInsertAtU6-26:sgRNA
UseCRISPRExpressionPlantPromoterU6-26Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Mito-CaNARi
Plasmid#64851PurposeMitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.DepositorInsertCaNARi
TagsCerulean, N-terminal 30 amino acids from DAKAP1, …ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR-NLS
Plasmid#118467Purposecp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.DepositorInsertsapphireAKAR-NLS
TagsNuclear localization signal (NLS)ExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR(T/A)
Plasmid#118406PurposeNegative-control mutant for ExRai-AKAR biosensor.DepositorInsertExRai-AKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-sapphireAKAR
Plasmid#118466Purposecp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.DepositorInsertLyn-sapphireAKAR
TagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianPromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
TagsCFP, N-terminal 27 amino acids from CYP450, and Y…ExpressionMammalianPromoterCMVAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-Kras
Plasmid#118408PurposePlasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-Kras
TagsC-terminal targeting sequence from KrasExpressionMammalianPromoterCMVAvailable SinceApril 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
dCas9-NLS-gs10-cGR2176
Plasmid#188258PurposePlasmid ensures constitutive expression of the C-terminal fragment of split GR2 (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminusDepositorInsertcGR2176
UseCRISPRExpressionMammalianAvailable SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-AKARev(T/A)
Plasmid#118479PurposeNegative-control mutant for RAB-AKARev biosensor.DepositorInsertRAB-AKARev(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR(T/A)
Plasmid#118473PurposeNegative-control mutant for blueCKAR biosensor.DepositorInsertblueCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ER-CaNARi
Plasmid#64852PurposeEndoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-CaNARi
TagsCerulean, N-terminal 27 amino acids from CYP450, …ExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFSynWSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209016PurposeOverexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cellsDepositorInsertsyt1 (Syt1 Rat)
UseLentiviralTagsmsfGFPExpressionMammalianMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETSyt1C2AB(80 JuxtaK-421)msGFP
Plasmid#209011PurposeRecombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFPDepositorInsertsyt1 (Syt1 Rat)
Tags6xHis and msfGFPExpressionBacterialMutationaa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…Available SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
nPPARg429-gs10-NLS-VPR
Plasmid#188266PurposePlasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnPPARg429 (PPARG Human)
ExpressionMammalianAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc3-bTrCP
Plasmid#20718DepositorInsertbeta-transducin repeat containing (BTRC) (Btrc Mouse)
TagsMyc3ExpressionMammalianMutation*Available SinceMay 1, 2009AvailabilityAcademic Institutions and Nonprofits only