Addgene

We narrowed to 16,163 results for: IGH@;

Showing: 201 - 220 of 16163 results

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pcDNA3.1-Pert Light chain

Plasmid

#216310

Purpose

Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Pert Light chain

Use

Affinity Reagent/ Antibody

Tags

Signal peptide

Expression

Mammalian

Mutation

Promoter

T7 promoter

Available since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Right-ND1-1397N-UGI

Plasmid

#183899

Purpose

Expresses TALED_Right-ND1-1397N-UGI in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

DDCBE for ND1 mutation

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX Tight Puro Snail

Plasmid

#101117

Purpose

Tet-On Snail

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

Snail cDNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Clathrin light chain

Plasmid

#83032

Purpose

Expresses sfCherry2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Clathrin light chain (CLTA Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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TALED_Right-mouse-ATP6-1397C-AD

Plasmid

#212954

Purpose

Expresses TALED_Right-mouse-ATP6-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

Insert

TALED_Right-mouse-ATP6-1397C-AD (ATP6 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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PB-tight-DDdCas9GFP-IRES-Neo

Plasmid

#102907

Purpose

PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9GFP-IRES-Neo

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

TRE-tight

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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APT3-PTEN (PTEN high)

Plasmid

#168006

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

PTEN-T3'PAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTrc99S-ssDsbA-TTlight-4xDQNAT

Plasmid

#128402

Purpose

Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin light chain domain

Use

Tags

4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

Expression

Bacterial

Mutation

Inactivating E234A mutation

Promoter

trc

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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CIp10 - YemVenus (OPT High)

Plasmid

#163122

Purpose

Expresses in Candida albicans

Depositor

Patrick Van Dijck

Article

Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

Insert

YemVenus OPT High

Use

Candida albicans expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE-TIGHT-Cx43-eYFP

Plasmid

#31807

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

Cx43 (GJA1 Human)

Use

Tags

eYFP

Expression

Mammalian

Mutation

Promoter

P-TIGHT

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pTRE-Tight-FLAG-CYLD-WT

Plasmid

#60030

Purpose

WT CYLD under control of Tet promoter with N-terminal FLAG tag

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only
EF1a-TALEN_HD (SPC right)

Plasmid

#126695

Purpose

right TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start site

Depositor

Darrell Kotton

Article

Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

Insert

human SPC right

Use

TALEN

Tags

NLS, truncated N term and TAL, FokI

Expression

Mammalian

Mutation

R451P in SPC

Promoter

EF1

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-Tight2-AkaLuc

Plasmid

#186186

Purpose

AAV vector packaging AkaLuciferase under the TRE promoter

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2022 May 19;13(1):2813. doi:

Insert

AkaLuc luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

TRE-Tight2

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397N-UGI

Plasmid

#187173

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397N-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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HK02-LP Clone 2 light chain

Plasmid

#192179

Purpose

Clone 2 light chain (HK02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 light chain (HK02)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTRE Tight ArchT-GFP

Plasmid

#79627

Purpose

Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

Depositor

Yasunori Hayashi

Article

Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

Insert

ArchT

Use

Tags

EGFP

Expression

Mammalian

Mutation

ArchT is a more sensitive version of Arch (archae…

Promoter

Tet responsive Ptight promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only
ND4-FusXTBE-Right TALE

Plasmid

#196497

Purpose

Expresses Right-side TALE-DddAtox Nterm half ND4-FusXTBE in mammalian cells

Depositor

Stephen Ekker

Article

Sabharwal et al CRISPR J. 2021 Dec;4(6):799-821. doi: 10

Insert

COX8A MTS-ND4 right TALE-G1397 DddA-C-UGI-ATP5B 3'UTR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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ET8-GPIba-high-6His

Plasmid

#180251

Purpose

High affinity mutant of human platelet membrane protein GP1b alpha

Depositor

Timothy Springer

Article

Jiang et al J Mol Biol. 2019 Mar 29;431(7):1380-1396

Insert

human GPIbalpha 1-290aa with mutation G233V and M239V (GP1BA Human)

Use

Tags

6His

Expression

Mammalian

Mutation

G233V and M239V

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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TALED_Right-stuffer-1397C-UGI

Plasmid

#187171

Purpose

original vector to make all constructs

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

Right-stuffer-1397C-UGI

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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KA1130_pPB-TREtight-cHA-IRESH2BmCherry

Plasmid

#124173

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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