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(112) pcDNA3.1-GFP-Flag-Nup62-EPEA

Plasmid

#168101

Purpose

GFP and Flag tagged Nup62 with EPEA nanobody tag

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

GFP-Flag-Nup62-EPEA

Tags

EPEA, Flag, and GFP

Expression

Mammalian

Promoter

T7/CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Cys-ELP(40nm)-LPETGG

Plasmid

#90475

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Cysteine and C-Terminal Sortase Recognition Sequence LPETGG

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

Cys-ELP(40nm)-LPETGG

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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AF1521-c003

Plasmid

#173111

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Tags

Nanoluc

Expression

Bacterial

Mutation

KH-M1-L192

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS10_1033_v1

Plasmid

#126860

Purpose

4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS10_1033_v1

Use

Synthetic Biology

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-2

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-6

Plasmid

#172771

Purpose

Bacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-6

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(40nm)-Cys

Plasmid

#91571

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Article

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(40nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo v2

Plasmid

#80033

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-3

Available Since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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(SIM)5

Plasmid

#126947

Purpose

Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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CapChR1_pmCherry-N1

Plasmid

#188031

Purpose

Expresses CapChR1-mCherry in mammalian cells

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1-mCherry

Tags

mCherry

Expression

Mammalian

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

CMV

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OgNLuc

Plasmid

#208851

Purpose

Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OgNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-2

Plasmid

#228984

Purpose

For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-GST-1

Plasmid

#228983

Purpose

For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLicC-MBP-APETx2

Plasmid

#72668

Purpose

Periplasmic expression of APETx2 in E.coli

Depositor

Glenn King

Article

Anangi et al Mar Drugs. 2012 Jul;10(7):1605-18. doi:

Insert

APETx2

Tags

His tag, MBP, MalE signal sequence for periplasmi…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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CMV500 A-C/EBP

Plasmid

#33352

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

C/EBP (Cebpa Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-2

Plasmid

#172768

Purpose

Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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L2383-FRB-GPA-NLuc

Plasmid

#222902

Purpose

Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-(SIM)3

Plasmid

#126955

Purpose

Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Tags

eGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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