We narrowed to 23,247 results for: crispr

1. pgRNA-bacteria

   Plasmid

   #44251

   Purpose

   Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   guide RNA (bacteria)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 (SpeI)

   Available since

   April 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tTRE, hPGK

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRS-XylS-dCas9

   Plasmid

   #220196

   Purpose

   mini-Tn7 dCas9 expression vector - XylS/pM promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   XylS/pM

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRS-LacI-dCas9

   Plasmid

   #220194

   Purpose

   mini-Tn7 dCas9 expression vector - LacI/pLac promoter

   Depositor

   Larry Halverson

   Article

   Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

   Insert

   Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Pseudomonas aeruginosa codon optimized

   Promoter

   LacI/pLac

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYPQ150

   Plasmid

   #69301

   Purpose

   Gateway entry vector with pcoCas9 (Plant codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   pcoCas9 (plant codon-optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   NLS fusion

   Promoter

   Available since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-sgRNA(MS2)

   Plasmid

   #102560

   Purpose

   Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-EFS-MCP-HALOnls

   Plasmid

   #121937

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   MCP-HALOnls
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas

   Plasmid

   #62225

   Purpose

   Constitutive expression of cas9 and inducible expression of lambda RED and sgR

   Depositor

   Sheng Yang

   Article

   Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

   Has Service

   Cloning Grade DNA

   Insert

   cas9
   

   Use

   CRISPR; Lambda red recombinase

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   native cas9 promoter

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0041-RanCas13b crRNA backbone

    Plasmid

    #103852

    Purpose

    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-RanCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD3-dU6:3gRNA

    Plasmid

    #49410

    Purpose

    expression of gRNA under control of the Drosophila U6:3 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-3:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-3

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJJB308

    Plasmid

    #107699

    Purpose

    AtU6 Promoter-LwaCas13a-gRNA Scaffold

    Depositor

    Daniel Voytas

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hUbC-dCas9-T2A-GFP

    Plasmid

    #53191

    Purpose

    Co-expresses human optimized S. pyogenes dCas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized dead Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SMVP-Cas9C

    Plasmid

    #80931

    Purpose

    Expresses Cas9C in mammalian cells.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9C
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-TO-dCas9-3XmCherry

    Plasmid

    #64108

    Purpose

    Expression of Sp dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti-EF1alpha-VP64-dCas9-p300_Blast

    Plasmid

    #192654

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-p300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A; H840A in Cas9

    Promoter

    EF1a

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hUbC-Cas9-T2A-GFP

    Plasmid

    #53190

    Purpose

    3rd generation transfer vector. Co-expresses human optimized S. pyogenes Cas9 and GFP

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Insert

    humanized Cas9 T2A GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    hUbC

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

    Plasmid

    #192650

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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