We narrowed to 26,289 results for: nes
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Plasmid#162335PurposeLentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection markerDepositorInsertS. pyogenes sgRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmTurqoise KIF1A shRNA
Plasmid#102849PurposeGenerates shRNA specific to Rat KIF1A along with cell fill pmTurqoiseDepositorAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRExpressionMammalianAvailable SinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMi[3xP3::mCherry-SV40, Plodia-FibL::mBaojin-P10]
Plasmid#240410PurposeFor Minos transgenesis in insect, expresses the 3xP3::mCherry eye/glia transgenesis, and the mBaoJin in Posterior Silk Glands (under the control of the Fibroin-L promoter from Plodia interpunctella)DepositorInsertsExpressionInsectPromoter3xP3 and Fibroin-L proximal promoter (Plodia inte…Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF5B
Plasmid#134625PurposeMammalian expression of mouse KIF5B 376-963DepositorInsertKIF5B (Kif5b Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 377 N-terminal residues.PromoterChicken Beta actinAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJ923
Plasmid#78313PurposeExpression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coliDepositorInsertSpCas9
TagsHA-2xNLS-mCherry-NLS and His6-MBP-TEVExpressionBacterialMutationhuman codon-optimized gene sequencePromoterT7Available SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PresentER-MSIIFFLPL (GFP)
Plasmid#102946PurposePresentER minigene encoding H2-Kb ligand MSIIFFLPL (GFP)DepositorAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRPromoterU6Available SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h1
Plasmid#86689PurposeFor transcription of of Suv4-20h1 mRNA preceded by an HA-tag in 5'DepositorAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-150
Plasmid#26310DepositorInserthsa-mir-150 (MIR150 Human)
ExpressionMammalianAvailable SinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
PUC19_C_tag
Plasmid#186659PurposeEmpty donor plasmid for C-terminal tag of 3xFlag-3xHA.DepositorInsertC-terminal 3xFlag-3xHA Tag
Tags3xFlag-3xHAExpressionInsectAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/MSCV-EGFP
Plasmid#82613PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseRetroviralTags3xFLAG tag and NLS (nuclear localization signal)Mutationhuman codon-optimized, D10A + H840APromoterLTRAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 mmu-mir-133a-1
Plasmid#26326DepositorInsertmmu-mir-133a-1 (Mir133a-1 Mouse)
ExpressionMammalianAvailable SinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyso-mCherry-Rheb(C181S)
Plasmid#192438PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.DepositorInsertLyso-mCherry-Rheb(C181S) (RHEB Human)
TagsFull-length LAMP1 and mCherryExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(C181S)
Plasmid#192437PurposeEncodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb Cys 181 mutated to Ser; abolishes C-terminal…PromoterCMVAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-124
Plasmid#26306DepositorInserthsa-mir-124 (MIR124-1 Human)
ExpressionMammalianAvailable SinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
L309-myc-seipin-WT
Plasmid#64061Purposemyc-tagged murine seipin (wild type); lentiviral constructDepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXB4 (human) pSG5
Plasmid#8527DepositorAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only