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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 2181 - 2200 of 16276 results

  1. pNB693

    Plasmid
    #60145
    Purpose
    Disintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available since
    Dec. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    60145-plasmid-map-sequence-id-96228

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  2. pNB689

    Plasmid
    #60133
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    MET17pr
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60133-plasmid-map-sequence-id-96189

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  3. pNB756

    Plasmid
    #60149
    Purpose
    Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    YeLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    LEU1pr
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60149-plasmid-map-sequence-id-96311

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  4. pNB752

    Plasmid
    #60155
    Purpose
    Green beetle luciferase with nuclear localization signal (NLS) and adjustable degron tag (ssrA), driven by ADE17 promoter.
    Depositor
    Nicolas Buchler
    Publication
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    GrLuc
    Tags
    ClpXP degron and Nuclear localization signal (NLS…
    Expression
    Yeast
    Promoter
    ADE17pr
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60155-plasmid-map-sequence-id-96323

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  9. pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant

    Plasmid
    #101215
    Purpose
    Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant K848A/K1003A/R1060A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    K848A, K1003A and R1060A
    Promoter
    T7
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    101215-plasmid-map-sequence-id-194634

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  11. pDNR-SpCas9-Gem

    Plasmid
    #80424
    Purpose
    Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining
    Depositor
    Sara Howden, Melissa Little
    Publication
    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-
    Insert
    SpCas9-Gem
    Use
    CRISPR
    Tags
    Geminin
    Mutation
    codon optimized for human
    Promoter
    T7
    Available since
    Aug. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    80424-plasmid-map-sequence-id-151441

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  17. pGolt-mCherry / pGolt3-mCherry

    Plasmid
    #73297
    Purpose
    Visualization of Dendritic Golgi Satellite
    Depositor
    Michael Kreutz
    Publication
    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    Tags
    ER export and mCherry
    Expression
    Mammalian
    Promoter
    CMV immediate early promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73297-plasmid-map-sequence-id-135341

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  20. pNICKclos2.0

    Plasmid
    #73228
    Purpose
    Genome editing for gene xylR (cbei-2385) in clostridium beijerinckii NCIMB 8052
    Depositor
    Sheng Yang
    Publication
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9 nickase
    sgRNA to xylR
    Use
    E.coli - clostridium shuttle vector
    Mutation
    D10A
    Promoter
    Pj23119 and Pthl
    Available since
    June 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    73228-plasmid-map-sequence-id-178086

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Showing: 2181 - 2200 of 16276 results