We narrowed to 24,400 results for: nes

1. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

   Plasmid

   #218508

   Purpose

   integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   mNeongreen
   

   Use

   Tags

   modified ssrA degradation tag

   Expression

   Bacterial

   Mutation

   deletion of MCS containing Plac_lacZa, introducti…

   Promoter

   P23

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX311-Cas9

   Plasmid

   #118018

   Purpose

   Constitutive expression of Cas9

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV phSyn1(S)-tdTomato-WPRE

   Plasmid

   #51506

   Purpose

   Can be used to generate AAV virus that will express tdTomato in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV5

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   phSyn1

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiGuide-Hygro-iRFP670

   Plasmid

   #99377

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Inserts

   S. pyogenes sgRNA cassette
   Hygro-P2A-iRFP670
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBa.GFP-KIF1Bbeta tail 387-1770

   Plasmid

   #134620

   Purpose

   Mammalian expression of mouse KIF1Bbeta 387-1170

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF1Bbeta (Kif1b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 386 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RHO-IntC-dCas9C-VPR-polyA

   Plasmid

   #166692

   Purpose

   Express C-terminal part of split dCas9-VPR

   Depositor

   Stylianos Michalakis

   Article

   Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

   Insert

   IntC-dCas9C-VPR
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   short human rhodopsin promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSR:MSMEG_0835:Dendra

   Plasmid

   #174923

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0835
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   pmycTetO

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PiggyBac-FLAG-mProser1-IRES-Blast

   Plasmid

   #226172

   Purpose

   Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

   Depositor

   Kasper Rasmussen

   Article

   Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

   Insert

   Proline Serine Rich protein 1 (Proser1 Mouse)
   

   Use

   Tags

   2X FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

   Plasmid

   #134611

   Purpose

   Mammalian expression of mouse KIF5A 378-1027

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 377 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSR:MSMEG_5070:Dendra

    Plasmid

    #167375

    Purpose

    C-term fluorescent fusion

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5070
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSR:MSMEG_5487:Dendra

    Plasmid

    #167440

    Purpose

    C-term fluorescent fusion

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5487
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSR:MSMEG_5447:Dendra

    Plasmid

    #167431

    Purpose

    C-term fluorescent fusion

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_5447
    

    Use

    Integrating mycobacterial expression vector attl5…

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLJM1-PHD2

    Plasmid

    #223552

    Purpose

    Lentivirus transfer plasmid for expression of full length human PHD2

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

    Insert

    PHD2 (EGLN1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-mCherry

    Plasmid

    #122957

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HOXB7 (human) FLAG pSP65

    Plasmid

    #8537

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    In vitro transcription/translation

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pMSR:MSMEG_0310:Dendra

    Plasmid

    #159051

    Purpose

    T7 expression of C-term fluorescent fusion in E. coli

    Depositor

    Keith Derbyshire, Joseph Wade

    Article

    Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

    Insert

    MSMEG_0310
    

    Use

    Integrating mycobacterial expression vector attl5

    Tags

    Gly-Ala Linker/Dendra2/1x Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pmycTetO

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 myc-Rab10

    Plasmid

    #208366

    Purpose

    Mammalian expression of myc-tagged human Rab10

    Depositor

    Suzanne Pfeffer

    Article

    Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

    Insert

    myc-Rab10 (RAB10 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CAG-NOTCH2NLB (N2NL-FL)-ires-EGFP

    Plasmid

    #122954

    Purpose

    Lentiviral expression of NOTCH2NLB full length under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NLB (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    Promoter

    CAG promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Flag-Jun4A-Myc

    Plasmid

    #47444

    Purpose

    mammalian expression of mutant c-Jun (four N-terminal residues phosphorylated by JNK are mutated into Alanines)

    Depositor

    Axel Behrens

    Article

    Aguilera et al Nature. 2011 Jan 13;469(7329):231-5. doi

    Insert

    Jun 4A mutant (Jun Mouse)
    

    Use

    Tags

    Flag and myc

    Expression

    Mammalian

    Mutation

    four N-terminal residues phosphorylated by JNK, s…

    Promoter

    CMV

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBa.GFP-KIF1Balpha tail 387-1150

    Plasmid

    #134619

    Purpose

    Mammalian expression of mouse KIF1Balpha 387-1150

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF1Balpha (Kif1b Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 386 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge