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mc2 neo EF1a

Plasmid

#206220

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CD4-sTb(C)-M13-GFP

Plasmid

#219784

Purpose

sTb(C) half of CaST for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Synapsin

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Expression

Mammalian and Yeast

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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C1-FT-mCitrine-EEVEE-CaaX

Plasmid

#155225

Purpose

MPAct with increased linker flexiblity

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with a codon optimized EEVEE and CaaX seq derived from Kras4b

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-mTagBFP2-2A

Plasmid

#102602

Purpose

Control lentivector. Coexpression of mTagBFP and gene of interest from CMV promoter

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pTEI039

Plasmid

#105428

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

LexA-VP16-ER (transcription factor: inducible expression; estradiol)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_07_01

Plasmid

#165014

Purpose

Expression of HLA-DRB1*07:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*07:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pHBV-X/EnhI-Luc

Plasmid

#71418

Purpose

To see HBV X promoter(overlaped EnhanceI) activity for luciferase assay

Depositor

Wang-Shick Ryu

Article

Ko et al J Virol. 2014 Dec;88(23):13689-98. doi:

Insert

HBV X promoter and enhancer I driving Luciferase expression

Use

Luciferase

Expression

Mammalian

Promoter

HBV X

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pOVC1_ScPH1-S_X

Plasmid

#131184

Purpose

Expresses ScPH1-S (Synechosystis PCC6803) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

ScPH1-S

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI071

Plasmid

#105376

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor CAB3 (At1g29910; 1000bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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p1D4-hrGFP II

Plasmid

#71317

Purpose

expression vector with 1D4 epitope added as a C-terminal tag, allowing for in vitro expression of any visual pigment or GPCR of interest.

Depositor

Belinda Chang

Article

Morrow et al Plasmid. 2010 Nov;64(3):162-9. doi: 10.1

Type

Empty backbone

Tags

1D4 epitope (TETSQVAPA) and IRES-hrGFPII

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only
pJOG299

Plasmid

#105360

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

promotor AP1 (At1g69120;1400bp)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mCit-IRES-Neo

Plasmid

#155230

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mCitrine sensor

Use

Lentiviral

Tags

mCitrine

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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mc2 neo

Plasmid

#206214

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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p5M-PR-A

Plasmid

#89124

Purpose

mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-A (PGR Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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Salsa-BC96-P1H12

Plasmid

#202937

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC96

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC96

Expression

Mammalian

Promoter

Tet Response Element (TRE)

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-L1-Reverse-Donor-Esp3I

Plasmid

#197845

Purpose

Donor vector to restore L1 destination via Esp3I (reverse orientation)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon hPGK

Plasmid

#206230

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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TTVTFLPTPSLILQT_epitope

Plasmid

#165021

Purpose

Expression of CMV UL44 (38-52) fused to CD74

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

CMV UL44 (38-52)

Use

Lentiviral

Tags

CD74 at N-term; cathepsin S cleavage site (GRWHTV…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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