Addgene

We narrowed to 2,956 results for: NAK

Showing: 2201 - 2220 of 2956 results

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AID-BE4max-P2A-GFP

Plasmid

#157948

Purpose

Mammalian expression of AID-BE4max-P2A-GFP

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

AID-BE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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A*33:01:01

Plasmid

#203288

Purpose

Overexpress HLA allele

Depositor

Catherine Wu, Derin Keskin

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*33:01:01 (HLA-A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PS

Plasmid

#136358

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)

Use

Tags

TRX-His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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904 pLNCX HA Akt1 K179M

Plasmid

#9007

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

K179M. Kinase dead.

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pYNL-C1_Rab11a

Plasmid

#65705

Purpose

Expresses YNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PdTK-CAG-mCh-[uBglII]

Plasmid

#107280

Purpose

puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

Depositor

Knut Woltjen

Article

Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

Insert

AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )

Use

Tags

Expression

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD3

Plasmid

#85650

Purpose

retroviral expression of human SMYD3

Depositor

Julien Sage

Article

Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

Insert

SMYD3 (SMYD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hEzh2-IRES-Hyg

Plasmid

#122375

Purpose

Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hEzh2-IRES-Hyg-pA

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shSLC2A1-1

Plasmid

#193702

Purpose

Constitutive lentiviral expression of SLC2A1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shSLC2A1-2

Plasmid

#193703

Purpose

Constitutive lentiviral expression of SLC2A1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

SLC2A1 (SLC2A1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Ldha

Plasmid

#175939

Purpose

knockout mouse Ldha

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Ldha gRNA (Ldha Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-WT-VLCAD-FLAG

Plasmid

#55823

Purpose

Expresses WT-VLCAD in mammalian cells

Depositor

Yoshimi Homma

Article

Kabuyama et al Am J Physiol Cell Physiol. 2010 Jan;298(

Insert

Very long-chain specific acyl-CoA dehydrogenase (ACADVL Human)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pMIR-21 Reporter

Plasmid

#71870

Purpose

Mammalian expression vector for the analysis of miR-21 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-21

Use

Tags

firefly luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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1071 pBabe puroL myr Akt T308A S473A

Plasmid

#9014

Depositor

William Sellers

Article

DiRenzo et al Cancer Res. 2002 Jan 1. 62(1):89-98.

Insert

Akt1 T308A S473A (AKT1 Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

T308A S473A

Promoter

Available since

Nov. 3, 2005

Availability

Academic Institutions and Nonprofits only
pCMV-LAMP1-mCherry

Plasmid

#214274

Purpose

Mammalian expression of LAMP1-mCherry (lysosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

LAMP1-mCherry (Lamp1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP9B-HA

Plasmid

#209214

Purpose

Mammalian expression of ATP9B

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP9B (ATP9B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pClneoMyc human MST2

Plasmid

#37022

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

MST2 (STK3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-OS

Plasmid

#136576

Purpose

Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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hGATA-1 K137R

Plasmid

#118353

Purpose

This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hGATA-1 (GATA1 Human)

Use

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

K137R

Promoter

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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