We narrowed to 15,156 results for: pET

1. pET24a-LaM4-1xTS

   Plasmid

   #162778

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
   

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-mAIM2-deltaPYD

   Plasmid

   #51535

   Purpose

   Express His6-tagged HIN domain of mAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   Aim2 (Aim2 Mouse)
   

   Tags

   His6 and T7

   Expression

   Bacterial

   Promoter

   lac operator

   Available Since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. TrmD_HaeIn/pET28b(+) N,C-tag

   Plasmid

   #12665

   Depositor

   SeWon Suh

   Article

   Kim et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. human TLNRD1 pET151

   Plasmid

   #159384

   Purpose

   Expresses human TLNRD1 in bacterial cells

   Depositor

   Ben Goult

   Article

   Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   Talin Rod Domain Containing Protein 1 (TLNRD1 Human)
   

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His10-Src42a

   Plasmid

   #126674

   Purpose

   Expression of His10-Src42a for purification from E. coli

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Src42a
   

   Expression

   Bacterial

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-21a(+)-IS200

   Plasmid

   #12604

   Depositor

   SeWon Suh

   Article

   Lee et al J Biol Chem. 2006 Feb 17. 281(7):4261-6.

   Insert

   IS200 transposase (SSO1474 Sulfolobus solfataricus)
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaG-9

   Plasmid

   #172742

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-9
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-p53 (Mouse 1-87)

   Plasmid

   #62061

   Purpose

   Expression of Mouse p53 (residues 1-87) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

   Insert

   p53 (Trp53 Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-87

   Promoter

   T7

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

10. C122-E16: CyPet

    Plasmid

    #162896

    Purpose

    Standard Gateway entry clone encoding CyPet fluorescent protein

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    CyPet fluorescent protein
    

    Use

    Synthetic Biology

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET28-FnCas12a-EP16

    Plasmid

    #183636

    Purpose

    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.

    Depositor

    Yunzi Luo

    Article

    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-

    Insert

    FnCas12a-EP16
    

    Use

    CRISPR

    Tags

    6×His Tag

    Expression

    Bacterial

    Mutation

    N607R, K613V, N617R, K180S, K660R, D616N

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET20b- hTPI Lys13Arg

    Plasmid

    #50726

    Purpose

    overexpression of the human TPI1 Lys13Arg allele in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    changed Lysine 13 to Argenine

    Promoter

    T7

    Available Since

    Feb. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-GST-Q11

    Plasmid

    #185790

    Purpose

    Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    GST-Q11
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-MBP-creM

    Plasmid

    #112238

    Purpose

    pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

    Depositor

    Emily Balskus

    Article

    Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

    Insert

    CreM
    

    Tags

    MBP

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. D5 gene in PETCON vector

    Plasmid

    #87626

    Purpose

    expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

    Depositor

    Sarel Fleishman

    Article

    Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

    Insert

    Designed allosteric Zn2+ and fluorescein binding antibody scFv D5
    

    Tags

    3x(G4S) Linker and cMyc tag on backbone

    Expression

    Yeast

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET30Xa-SBL1(160S)

    Plasmid

    #66872

    Purpose

    Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)
    

    Tags

    Histag from pET30Xa-LIC

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7lac

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. FKBP-YPet-RanGAP*

    Plasmid

    #175245

    Purpose

    Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    RANGAP1 (RANGAP1 Human)
    

    Expression

    Bacterial

    Mutation

    Amino acid 562 F to A

    Promoter

    tac

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a BCP1 NTase

    Plasmid

    #195245

    Purpose

    Expresses phage BCP1 NTase

    Depositor

    Douglas Feldman

    Article

    Ho et al Cell Rep. 2023 Mar 22;42(4):112305. doi:

    Insert

    BCP1 phage NTase
    

    Tags

    His6 and SBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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