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pAK_lentiguide_DR30_Puro

Plasmid

#134839

Purpose

Lentiviral expression CasRx guideRNA

Depositor

Michael Yaffe

Insert

CasRx guideRNA backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX_970_puro_DEST_V5_BFP

Plasmid

#45297

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

BFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
iC13 PB-TO-oNgn2-BFP-puro

Plasmid

#204733

Purpose

Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Optimized neurogenin-2

Expression

Mammalian

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SFFV-NFE2L1-Puro

Plasmid

#181917

Purpose

Lentiviral plasmid that directs the expression of NFE2L1/Nrf1 in mammalian cells.

Depositor

Scott Dixon

Publication

Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

NFE2L1 (NFE2L1 Human)

Use

Lentiviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

SFFV

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLD-puro-Cc-TARDBP-WT_VA

Plasmid

#141327

Purpose

Lentiviral vector for expression of C-terminally VA-tagged human TARDBP-WT in mammalian cells

Depositor

Mohan Babu

Publication

Pourhaghighi et al Cell Syst. 2020 Apr 22;10(4):333-350.e14

Insert

TARDBP-WT (TARDBP Human)

Use

Lentiviral

Tags

VA tag (Beacon-2XStrep-6XHis-2XTEV-3XFLAG)

Expression

Mammalian

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-VHL W117R-pBabe-puro

Plasmid

#19239

Depositor

William Kaelin

Publication

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tryptophan 117 to Arginin

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBabe-Puro-RalA-V23

Plasmid

#15252

Depositor

William Hahn

Publication

Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

Insert

v-ral simian leukemia viral oncogene homolog A (RALA Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed 23rd amino acid to valine

Available since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLJM1-EGFP-BarcodeV1

Plasmid

#78633

Purpose

GESTALT V1 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT target V1

Use

Lentiviral

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSCAR_sgRNA_puro-mKate-lox5171

Plasmid

#162077

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLX302 MX1-V5 puro

Plasmid

#158640

Purpose

Lentiviral expression vector for constitutive expression of V5-tagged MX1.

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

Mx1 (MX1 Human)

Use

Lentiviral

Tags

V5

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pWPI-IRES-Puro-Ak

Plasmid

#154984

Purpose

Lentiviral vector for expressing mammalian proteins

Depositor

Sonja Best

Type

Empty backbone

Use

Lentiviral

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Publication

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-puro HA-Atg14*

Plasmid

#38265

Depositor

Noboru Mizushima

Publication

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pRetroSuper shSmad4J puro

Plasmid

#19152

Depositor

Joan Massague

Publication

Padua et al Cell. 2008 Apr 4. 133(1):66-77.

Insert

small hairpin RNA against SMAD family member 4 (SMAD4 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only
pLenti-FNLSNG-P2A-Puro

Plasmid

#136900

Purpose

Lentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)

Depositor

Lukas Dow

Publication

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

FNLSNG

Use

Lentiviral

Tags

FLAG-NLS and NLS

Mutation

L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T1337R

Promoter

EF1s

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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OCT4 GFP puro Donor 3

Plasmid

#22211

Depositor

Rudolf Jaenisch

Publication

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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HA-VHL Y98N-pBabe-puro

Plasmid

#19236

Depositor

William Kaelin

Publication

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 98 to Asparagin

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBABE puro mouse IRS-2

Plasmid

#11371

Depositor

Ronald Kahn

Publication

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-2 (Irs2 Mouse)

Use

Retroviral

Expression

Mammalian

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLV-Puro_hCOL4A4

Plasmid

#198099

Purpose

lentiviral expression of hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A4 (COL4A4 Human)

Use

Lentiviral

Promoter

EF1a

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pN-PURO-PTP

Plasmid

#51711

Purpose

contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

Depositor

Arthur Gunzl

Publication

Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

Type

Empty backbone

Use

T. brucei genome integration vectors

Tags

PTP (ProtC-TEV-ProtA)

Available since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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